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Biochemical study and digestion profile of olive oil by LipBK: Revealing the potential applications of a new acid/broad thermal range true lipase.

Int J Biol Macromol

January 2025

Department of Agricultural and Environmental Biotechnology, São Paulo State University (UNESP), School of Agricultural and Veterinarian Sciences, Jaboticabal, São Paulo, Brazil; Institute of Bioenergy Research (IPBEN), Jaboticabal, São Paulo, Brazil. Electronic address:

This study characterized a novel bacterial lipase with high biotechnological potential, focusing on industrial and environmental applications. Bacterial isolates were screened using olive oil as a substrate, and the strain with the highest hydrolytic halo was identified as Burkholderia sp. via 16S rRNA analysis.

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Necrotizing enterocolitis (NEC) is a devastating disease of the neonatal gastrointestinal tract. Volatile organic compounds (VOCs), odoriferous compounds released as a byproduct of bacterial metabolism, can be used as a proxy for gut health. We hypothesized that patients with NEC would have different microbial profiles and elicit different VOC signatures as assessed by gas chromatography/mass spectrometry (GC/MS) or an electronic nose compared to controls.

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Low-carbon fuels, emitting less carbon than fossil fuels, are proposed to help in the transition to a sustainable, decarbonized transport sector. The new biofuels being studied and developed in this context include hydrotreated vegetable oils (HVO). Its chemical composition, which is the same as fossil diesel (primarily composed of linear chain hydrocarbons C12-C24), makes HVO (more homogeneous mixtures of paraffinic hydrocarbons C10-C20, containing no sulfur or aromatics) a fuel with slightly lower density than fossil diesel due to these characteristics.

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The occurrence of off-flavor in osmanthus absolutes has emerged as a significant concern that could hinder its broad market acceptance and associated economic development. In this study, key off-flavor molecules in industrial osmanthus absolute were identified through sensomics and chemometric approaches. A group of 10 off-flavor (OF) samples, eliciting smoky/phenolic, sweaty/sour, and spicy odors, were compared with 10 pleasant aroma (PA) samples through various analyses, including overall aroma assessment, comprehensive chemical profiling, aroma extract dilution analysis (AEDA), and orthogonal partial least-squares-discriminant analysis (OPLS-DA).

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Alkylated polycyclic aromatic hydrocarbons (PAHs) are abundant constituents of many PAH mixtures and contribute to risk at contaminated sites. Despite their abundance, the movement of alkylated PAHs remains understudied relative to unsubstituted PAHs. In the present study, passive sampling devices were deployed in the air, water, and sediments at 11 locations across multiple seasons to capture spatial and temporal variability in the abundance and movement of alkylated PAHs at a Brownsfield creosote site in Oregon, USA.

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