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Dokl Biochem Biophys
January 2025
Voronezh State University, Voronezh, Russia.
Creation and long-term in vitro maintenance of valuable genotype collection is one of the modern approach to conservation of valuable gene pool of woody plants. However, during prolonged cultivation, genetic variability of cells and tissues may accumulate and lead to the loss of valuable characteristics of parental plants. It is therefore important to assess the genetic (including cytogenetic) stability of collection clones.
View Article and Find Full Text PDFJMIR Form Res
January 2025
ICMR-National Institute for Research in Digital Health and Data Science, Ansari Nagar, New Delhi, 110029, India, 91 7840870009.
Background: Verbal autopsy (VA) has been a crucial tool in ascertaining population-level cause of death (COD) estimates, specifically in countries where medical certification of COD is relatively limited. The World Health Organization has released an updated instrument (Verbal Autopsy Instrument 2022) that supports electronic data collection methods along with analytical software for assigning COD. This questionnaire encompasses the primary signs and symptoms associated with prevalent diseases across all age groups.
View Article and Find Full Text PDFJ Biomed Opt
January 2025
Texas A&M University, Department of Biomedical Engineering, College Station, Texas, United States.
Significance: Accurate values of skin optical properties are essential for developing reliable computational models and optimizing optical imaging systems. However, published values show a large variability due to a variety of factors, including differences in sample collection, preparation, experimental methodology, and analysis.
Aim: We aim to explore the influence of storage conditions on the optical properties of the excised skin from 400 to 1100 nm.
BMJ Open
January 2025
British Heart Foundation Cardiovascular Epidemiology Unit, Department of Public Health and Primary Care, University of Cambridge, Cambridge, UK.
Purpose: Bangladesh has experienced a rapid epidemiological transition from communicable to non-communicable diseases (NCDs) in recent decades. There is, however, limited evidence about multidimensional determinants of NCDs in this population. The BangladEsh Longitudinal Investigation of Emerging Vascular and nonvascular Events (BELIEVE) study is a household-based prospective cohort study established to investigate biological, behavioural, environmental and broader determinants of NCDs.
View Article and Find Full Text PDFCureus
December 2024
Department of Clinical Research, University of San Luis Potosí, San Luis Potosí, MEX.
Background: In large-scale molecular studies, a protocol that generates high yields and quality DNA for future polymerase chain reaction (PCR) assays is needed. The collection of buccal cells by cytobrush may represent an efficient, noninvasive, and inexpensive method for obtaining genetic material from school populations. The aim of this study was to develop a method to obtain genomic DNA from buccal cells of schoolchildren, and the DNA was extracted immediately after collecting the buccal cell samples and after storing the samples for 8 months at -20 °C to establish the feasibility of the method for epidemiological studies.
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