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Function: require_once
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http://dx.doi.org/10.1007/978-1-4615-9101-6_16 | DOI Listing |
Immunol Cell Biol
December 1991
Division of Cell Biology, John Curtin School of Medical Research, Australian National University, Canberra.
Previous studies have shown that certain sulfated polysaccharides, such as dextran sulfate (DxS), can induce a rapid and sustained increase in the free intracellular calcium ion concentration ([Ca2+]i) of thymocytes, whereas they cannot induce a change in the [Ca2+]i of peripheral lymphocytes. This study examined this phenomenon in more detail and clearly demonstrated that only immature thymocytes can respond to DxS. Cortisone-resistant thymocytes failed to exhibit an increase in [Ca2+]i when exposed to DxS.
View Article and Find Full Text PDFDev Comp Immunol
July 1991
Basic Research Laboratories, Ajinomoto Co., Inc., Kawasaki, Japan.
Interleukin 6 (IL-6) and interleukin 1 (IL-1) were compared as to their ability to induce the proliferation of distinct thymocyte subpopulations. IL-6 functions as a costimulator in IL-1- or IL-2-induced proliferation of adult double-negative (DN) thymocytes, whereas IL-6 alone failed to induce a significant level of proliferation. However, IL-6 alone induced significant proliferation of mature cortisone-resistant thymocytes, whereas IL-1 did not.
View Article and Find Full Text PDFEur J Immunol
March 1990
Laboratoire de Physiopathologie du Développement, CNRS URA 230, Paris, France.
Murine thymocytes are shown to possess at least three well-defined exo-N-aminopeptidase activities on their surface. One of them cleaves the prolyl bond in the synthetic dipeptide nitroanilide Gly-Pro-pNA (Km 0.95 mM and Vmax 8 nmol/h at pH 7.
View Article and Find Full Text PDFJ Immunol
September 1989
Department of Immunology, DNAX Research Institute of Cellular and Molecular Biology, Palo Alto, CA 94304.
The frequency and capacity for clonal expansion of several murine thymocyte subpopulations responsive to various IL (fetal day 15, and adult CD4-8-, CD4+8- and CD4-8+) were investigated using a single-cell limiting-dilution cell culture system without filler cells. This assay requires the presence of PMA and ionomycin. The main conclusions of these studies are the following: 1) IL-4 is a better growth factor than IL-2 for immature thymocytes (fetal day 15 or adult CD4-8-).
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