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Synthesis of the conjugation-ready β-mannosamine-containing -antigen repeat from O14.
Org Biomol Chem
January 2025
Sweet Lab, Department of Chemical Sciences, Indian Institute of Science Education and Research Kolkata, Mohanpur, Nadia 741246, India.
Chemical synthesis of the trisaccharide repeating unit of the -antigen from has been accomplished through a linear strategy. The reducing end 1,2- mannosamine unit has been achieved through the azide inversion of the 2-OH position of a suitably protected glucose moiety. The crucial ()-3-hydroxybutyric acid is inserted successfully at the last stage through EDC-HOBt coupling.
View Article and Find Full Text PDFConstruction and analysis of the immune effect of subunit vaccines based on the flagellin FlaB and FlaC of Vibrioharveyi.
Fish Shellfish Immunol
January 2025
Sanya Institute of Breeding and Multiplication, School of Marine Biology and Fisheries, Collaborative Innovation Center of Marine Science and Technology, Hainan University, Hainan, China; Engineering Research Center of Hainan Province for Blue Carbon and Coastal Wetland Conservation·and·Restoration, China; International Joint Research Center of Hainan Province for Blue Carbon and Coastal Wetland, China. Electronic address:
Vibrio harveyi is a common sort of pathogenic bacterium in marine, which annually gives rise to huge financial losses in aquaculture industry. Flagellin is one of the important virulence factors for bacteria, but meanwhile it is also a preferable vaccine candidate. In this study, we have identified and analyzed two flagellin antigens of V.
View Article and Find Full Text PDFProteomic Analysis of the Fish Pathogen Strain Vo-LM-18 and Its Outer Membrane Vesicles.
Animals (Basel)
December 2024
Interdisciplinary Center for Aquaculture Research (INCAR), Universidad Andrés Bello, Viña del Mar 2531015, Chile.
is the causative agent of atypical vibriosis in salmonids cultured in Chile. While extensive research provides insights into through phenotypic, antigenic, and genetic typing, as well as various virulence mechanisms, proteomic characterization remains largely unexplored. This study aimed to advance the proteomic knowledge of Chilean Vo-LM-18 and its OMVs, which have known virulence.
View Article and Find Full Text PDFBacteria use exogenous peptidoglycan as a danger signal to trigger biofilm formation.
Nat Microbiol
January 2025
Biozentrum, University of Basel, Basel, Switzerland.
For any organism, survival is enhanced by the ability to sense and respond to threats in advance. For bacteria, danger sensing among kin cells has been observed, but the presence or impacts of general danger signals are poorly understood. Here we show that different bacterial species use exogenous peptidoglycan fragments, which are released by nearby kin or non-kin cell lysis, as a general danger signal.
View Article and Find Full Text PDFSurface remodeling and inversion of cell-matrix interactions underlie community recognition and dispersal in Vibrio cholerae biofilms.
Nat Commun
January 2025
Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT, USA.
Biofilms are ubiquitous surface-associated bacterial communities embedded in an extracellular matrix. It is commonly assumed that biofilm cells are glued together by the matrix; however, how the specific biochemistry of matrix components affects the cell-matrix interactions and how these interactions vary during biofilm growth remain unclear. Here, we investigate cell-matrix interactions in Vibrio cholerae, the causative agent of cholera.
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