Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Subcellular NAD pools are interconnected and buffered by mitochondrial NAD.
Nat Metab
December 2024
Department of Biomedicine, University of Bergen, Bergen, Norway.
The coenzyme NAD is consumed by signalling enzymes, including poly-ADP-ribosyltransferases (PARPs) and sirtuins. Ageing is associated with a decrease in cellular NAD levels, but how cells cope with persistently decreased NAD concentrations is unclear. Here, we show that subcellular NAD pools are interconnected, with mitochondria acting as a rheostat to maintain NAD levels upon excessive consumption.
View Article and Find Full Text PDFABCG1 orchestrates adipose tissue macrophage plasticity and insulin resistance in obesity by rewiring saturated fatty acid pools.
Sci Transl Med
December 2024
Sorbonne Université, INSERM, Foundation for Innovation in Cardiometabolism and Nutrition (ICAN), UMR_S1166, F-75013 Paris, France.
The mechanisms governing adipose tissue macrophage (ATM) metabolic adaptation during diet-induced obesity (DIO) are poorly understood. In obese adipose tissue, ATMs are exposed to lipid fluxes, which can influence the activation of specific inflammatory and metabolic programs and contribute to the development of obesity-associated insulin resistance and other metabolic disorders. In the present study, we demonstrate that the membrane ATP-binding cassette g1 (Abcg1) transporter controls the ATM functional response to fatty acids (FAs) carried by triglyceride-rich lipoproteins, which are abundant in high-energy diets.
View Article and Find Full Text PDFAnalysis of stallion spermatozoa metabolism using Agilent Seahorse XFp Technology.
Anim Reprod Sci
December 2024
Department of Veterinary Medical Sciences, Alma Mater Studiorum-University of Bologna, Bologna, Italy.
Sperm metabolism consists of a sophisticated network of biochemical reactions and varies between species, resulting in different metabolic strategies for ATP production to maintain sperm functionality. ATP can be produced through glycolysis or in the mitochondria by oxidative phosphorylation (OXPHOS). Since OXPHOS is the predominant metabolic pathway in horses spermatozoa, various assessments of mitochondrial activity are used to evaluate fertility, utilizing techniques such as fluorescent probes analysed via microscopy or flow cytometry, and polarographic electrode assays to measure current flow in response to an applied voltage.
View Article and Find Full Text PDFInvestigating the Antibacterial Effect of a Novel Gallic Acid-Based Green Sanitizer Formulation.
Foods
October 2024
Institute of Postharvest and Food Sciences, Agricultural Research Organization, The Volcani Institute, 68 HaMaccabim Road, P.O. Box 15159, Rishon LeZion 7505101, Israel.
The purpose of the present study was to investigate the mechanism of action of our newly developed green sanitizer formulation comprising a natural phenolic compound, gallic acid (GA), strengthened by the Generally Recognized as Safe (GRAS) materials hydrogen peroxide (HO) and DL-lactic acid (LA). Combining 8 mM GA with 1 mM HO resulted in an abundant generation of reactive oxygen species (ROS) and a bactericidal effect towards Gram-negative (, , and ) and Gram-positive () bacteria (4 to 8 log CFU mL reduction). However, the exposure to this dual formulation (DF) caused only a modest 0.
View Article and Find Full Text PDFPurine limitation prevents the exogenous pyridoxal 5'-phosphate accumulation of mutants.
Microbiol Spectr
October 2024
Department of Microbiology, University of Georgia, Athens, Georgia, USA.
Unlabelled: YggS belongs to the highly conserved pyridoxal 5'-phosphate (PLP) binding protein family (COG0325) that is found in all domains of life. Though no precise biochemical activity or molecular mechanism has been determined for this protein, an involvement in vitamin B homeostasis has been demonstrated in multiple organisms. In , loss of YggS results in altered B vitamer pools, including an accumulation of PLP in the growth medium.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!