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Extraction of Essential Oils and Terpene Volatiles from Plants and Identification by GC-MS-Based Techniques.
Methods Mol Biol
January 2025
Department of Cell and Metabolic Biology, Leibniz Institute of Plant Biochemistry, Halle, Germany.
The extraction of plant essential oils (EOs) and analysis by gas chromatography coupled to mass spectrometry (GC-MS) are standard methods when studying aromatic plants and the chemical composition of EOs. Here, two simple methods for the extraction of EO compounds from leaves of Thymus vulgaris are described. Organic solvent extraction and solid-phase microextraction (SPME), respectively, are used and the results of the GC-MS analyses are compared.
View Article and Find Full Text PDFTargeting antibodies dissociate from drug delivery liposomes during blood circulation.
J Control Release
January 2025
Biotherapeutic Engineering and Drug Targeting, Department of Health Technology, Technical University of Denmark (DTU), Kgs. Lyngby, Denmark. Electronic address:
Despite three decades of intense research, active targeting of liposomes have not been successfully achieved in a clinical setting. A potential explanation is that the clinically used liposomes lose their targeting abilities upon circulation. Here, we investigated if DSPE-PEG anchored antibody-based targeting ligands dissociate from clinically relevant drug delivery liposomes during circulation in mice.
View Article and Find Full Text PDFObesity phenotypes and dyslipidemia in adults from four African countries: An H3Africa AWI-Gen study.
PLoS One
January 2025
Faculty of Health Sciences, Sydney Brenner Institute for Molecular Bioscience, University of the Witwatersrand, Johannesburg, Johannesburg.
Introduction: The contribution of obesity phenotypes to dyslipidaemia in middle-aged adults from four sub-Saharan African (SSA) countries at different stages of the epidemiological transition has not been reported. We characterized lipid levels and investigated their relation with the growing burden of obesity in SSA countries.
Methods: A cross-sectional study was conducted in Burkina Faso, Ghana, Kenya and South Africa.
Analysis of Total, Free, and Esterified Sterols and Triterpene Alcohols in Edible Oils using a Gas Chromatography-Flame Ionization Detector.
J Oleo Sci
January 2025
Faculty of Food and Agricultural Sciences, Fukushima University.
Sterols and triterpene alcohols exist in free and esterified forms in edible oils. To date, only few studies have determined the content of free or esterified sterols and triterpene alcohols using gas chromatography-flame ionization detection (GC-FID). In this study, analytical conditions were optimized using free and esterified sterol standards.
View Article and Find Full Text PDFComprehensive analysis of flower extracts: phytochemical composition and toxicity in zebrafish embryos.
Nat Prod Res
January 2025
Programa de Pós-Graduação em Farmacologia, Universidade Federal de Santa Maria (UFSM), Santa Maria, Brazil.
(L.) R. Br.
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