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Identification of novel cytoskeleton protein involved in spermatogenic cells and sertoli cells of non-obstructive azoospermia based on microarray and bioinformatics analysis.
BMC Med Genomics
January 2025
Department of Surgery, Faculty of General of Medicine, Koya University, Koya, Kurdistan Region - F.R., KOY45, Iraq.
Background: During mammalian spermatogenesis, the cytoskeleton system plays a significant role in morphological changes. Male infertility such as non-obstructive azoospermia (NOA) might be explained by studies of the cytoskeletal system during spermatogenesis.
Methods: The cytoskeleton, scaffold, and actin-binding genes were analyzed by microarray and bioinformatics (771 spermatogenic cellsgenes and 774 Sertoli cell genes).
Morphokinetic Analyses of Fishing Cat-Domestic Cat Interspecies Somatic Cell Nuclear Transfer Embryos Through A Time-Lapse System.
Animals (Basel)
January 2025
Endangered Species Conservation via Assisted Reproduction (ESCAR) Lab, Institute of Molecular and Cell Biology (IMCB), Agency for Science, Technology and Research (A*STAR), 61 Biopolis Drive, Proteos, Singapore 138673, Singapore.
A time-lapse live embryo monitoring system provides a powerful approach to recording dynamic developmental events of cultured embryos in detail. By obtaining continuous short-interval images, blastocyst formation can be predicted and embryos can be selected. The objective of this study was to investigate the morphokinetic parameters of fishing cat-domestic cat interspecies somatic cell nuclear transfer (iSCNT) embryos from one-cell to blastocyst stages, and in particular, the cleavage patterns of the first division in iSCNT and IVF embryos, as these play a central role in euploidy.
View Article and Find Full Text PDFRing strain governs transmetalation behaviour at a tucked-in iron complex.
Chem Commun (Camb)
January 2025
Department of Chemistry, Western University, 1151 Richmond St, London, ON, N8K 3G6, Canada.
Studies that independently investigate [M]-C transmetalation reactions using two different metals are uncommon and yet understanding this reactivity is important to unlocking new synthetic approaches and product classes. Here, we show that the strained [Fe]-C complex, [(η-CMe-CH)Fe(diphosphine)] undergoes transmetalation with rhodium(I) and iridium(I) diolefin salts, leading to rapid Fe-C(sp) bond cleavage and M-C(sp) (M = Rh or Ir) bond generation.
View Article and Find Full Text PDFAnterior Cruciate Ligament Mechanical Response to Load in the Setting of Changes to the Medial Meniscus.
Bioengineering (Basel)
January 2025
Department of Orthopaedic Surgery, University of Illinois at Chicago, Chicago, IL 60612-7342, USA.
The anterior cruciate ligament (ACL) is a major ligament in the knee joint, and its function is crucial for both the movement and stability of the knee. Our research takes a novel approach by investigating the effect of meniscus tears on the ACL, how such tears will impact the stress on the ACL, and its overall compensation in response to the changes in the meniscus. : This study aims to investigate how the ACL compensates for the change in knee joint stability and contact pressures due to partial horizontal cleavage tears (HCTs) in the meniscus, such as partial meniscectomy and partial transplantation on knee joint stability and contact pressures.
View Article and Find Full Text PDFPyro-Thermolysis Pattern Analysis of Selenopeptide in Selenium-Enriched Rice Based on Two-Dimensional Dietary Kinetics Evolution.
J Agric Food Chem
January 2025
Ankang Research and Development Center for Se-Enriched Products, Ankang 725000, China.
Selenopeptides can be ideal dietary selenium (Se) supplements for humans. Currently, rice is not used much as a source of selenopeptides. Here, we executed the selenopeptidomics analysis of selenium-enriched rice protein hydrolysates using the full MS-dd-MS2 acquisition method and identified selenopeptides, including L{Se-Met}AK and other selenopeptides.
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