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Probiotic viability in the gastrointestinal tract in a randomised placebo controlled trial: combining molecular biology and novel cultivation techniques.
Benef Microbes
January 2025
Innovative Research Institute, Morinaga Milk Industry Co., Ltd., 1-83, 5-Chome, Higashihara, 252-8583, Zama-City, Kanagawa, Japan.
Understanding the viability of ingested probiotics within the gastrointestinal tract is essential for evaluating their efficacy and deciphering their mechanisms of action. Detecting Bifidobacterium longum subspecies longum BB536 is particularly challenging owing to its indistinguishability from the naturally abundant B. longum species in the human gut.
View Article and Find Full Text PDFProbiotics fermentation enhanced the bioactive properties of water extract and improved regulation ability of gut microbiota.
Food Chem X
January 2025
Key Laboratory of Microbiology and Parasitology of Education Department of Guizhou, School of Basic Medical Science, Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guiyang 550025, PR China.
This study investigated the probiotic potential of fermented beverages derived from (). Three different beverages were prepared by fermenting water extract with A6-3 ( A6-3), A27-1 ( A27-1), or both for 48 h. The results demonstrated that bioactive compounds from promoted the growth of these two probiotics and preserved their viability for at least 28 days at 4 °C.
View Article and Find Full Text PDFChanges in Human Colonic Microbiota Promoted by Synbiotic Açai Juice Composed of Gluco-Oligosaccharides, Dextran, and NRRL B-41408.
Foods
December 2024
Food Engineering Department, Federal University of Ceara, Fortaleza 60440-900, CE, Brazil.
The present study evaluates the effects of açai juice containing gluco-oligosaccharides and dextran, fermented by NRRL B-41408 (synbiotic juice), on the human fecal microbiota. The juice is subjected to simulated digestion and fecal fermentation after production and 42 days of refrigerated storage. High throughput 16S rRNA sequencing and HPLC are used to identify the bacterial cells and metabolites.
View Article and Find Full Text PDFLong-term stability and efficacy of frozen fecal microbiota transplant (FMT) product at 24 months.
Dig Liver Dis
December 2024
Department of Surgery, Oncology and Gastroenterology (DISCOG), University Hospital of Padua, Padua, Italy; Gastroenterology Unit, Azienda Ospedale Università di Padova, Padova, Italy. Electronic address:
Background: Freezing donor fecal microbiota has improved fecal microbiota transplantation (FMT) for recurrent C. difficile infection (CDI), achieving short-term effectiveness similar to fresh-samples. Research shows frozen fecal matter remains effective for up to 12-months at -80 °C.
View Article and Find Full Text PDFEnhancing the functionality of yogurt: Impact of exotic fruit pulps addition on probiotic viability and metabolites during processing and storage.
Food Res Int
November 2024
Department of Food Science and Nutrition, Faculty of Food Engineering, University of Campinas, Campinas, SP, Brazil. Electronic address:
The effect of exotic fruit pulp (araçá, cagaita, cambuci, grumixama, blueberry, and uvaia) on probiotic viability during storage and after gastrointestinal tract (GIT) simulation, in vitro biological activity, total phenolics (TPC) and flavonoids content, and the production of metabolic products of microbial in probiotic yogurt were investigated. During the storage period (28 days), probiotic strain populations remained high (above 6 log CFU/g), while after the GTI simulation, L. acidophilus and B.
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