Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/0005-2744(73)90415-4 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Application of two novel anionic peroxidases from L. var roots in labeling antibodies and developing an enzyme-linked immunosorbent assay.
Heliyon
January 2025
Department of Clinical Biochemistry, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
HRP, or horseradish peroxidase, is a reporter enzyme with extensive use in biotechnological applications. We previously reported the purification and characterization of two anionic peroxidases from L. var (black radish) roots.
View Article and Find Full Text PDFIsolation and characterization of the midgut microbiota of Aedes albopictus to identify suitable candidates for paratransgenesis.
Eur J Public Health
January 2025
Malaria and Vector Research Group (MVRG), Biotechnology Research Center (BRC), Pasteur Institute of Iran, Tehran, Iran.
Aedes albopictus is a widely recognized carrier of various pathogens. Its resilient characteristics enable it to easily spread across diverse climates. The microbiota in the midgut of mosquitoes plays a crucial role in the interactions between the host and pathogens and can either enhance or reduce the ability of the insect to transmit diseases.
View Article and Find Full Text PDFPurification and characterization of a thermophilic NAD-dependent lactate dehydrogenase from Moorella thermoacetica.
FEBS Open Bio
January 2025
Department of Molecular Microbiology & Bioenergetics, Institute of Molecular Biosciences, Johann Wolfgang Goethe University, Frankfurt am Main, Germany.
Oxidation of lactate under anaerobic dark fermentative conditions poses an energetic problem. The redox potential of the lactate/pyruvate couple is too electropositive to reduce the physiological electron carriers NAD(P) or ferredoxin. However, the thermophilic, anaerobic, and acetogenic model organism Moorella thermoacetica can grow on lactate but was suggested to have a NAD-dependent lactate dehydrogenase (LDH), based on enzyme assays in cell-free extract.
View Article and Find Full Text PDFA Pyrrole-Fused Nanographene and its Edge-Perchlorinated Derivative Featuring a Corannulene Core and Five N-doped Heptagons.
Angew Chem Int Ed Engl
January 2025
Xiamen University, Department of Chemistry, CHINA.
A pyrrole-fused analogue of warped nanographene, designated as deca-nitrogen doped 'WNG' (azaWNG), was synthesized through the annular fusion of decapyrroylcorannulene. The resulting azaWNG exhibited extremely limited solubility in common organic solvents and was characterized solely by mass spectrometry. Theoretical calculations revealed that azaWNG has a sunflower-like molecular structure with electron-deficient corannulene as the core and electron-rich pyrrole as the petals, demonstrating a significantly narrower energy gap compared to all-carbon WNG.
View Article and Find Full Text PDFSibanin, a novel black fly-derived Kunitz protease inhibitor, prevents thrombus formation in mice by anticoagulation-antiplatelet duality.
Int J Biol Macromol
January 2025
School of Basic Medical Sciences, Kunming Medical University, Kunming 650500, Yunnan, China. Electronic address:
Most Kunitz inhibitors exhibit serine protease inhibitory activity, but limited information is available on the regulation of platelet function. Herein, we report the purification and characterization of a novel single Kunitz domain inhibitor (Sibanin) from the salivary glands of the black fly Simulium bannaense. Recombinant Sibanin prolonged activated partial thromboplastin time and prothrombin time, and exhibited high-affinity binding to FXa and elastase with a KD of 5.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!