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How Can RuBisCO Be Released from the Mesophyll Cells of Green Tea Residue?
J Agric Food Chem
December 2024
Ecofood Institute, College of Biological Science and Engineering, Fuzhou University, Fuzhou 350108, China.
Article Synopsis
- This study focuses on the release of RuBisCO from green tea residue mesophyll cells (TRMCs), which has not been successfully achieved before.
- Researchers used a combination of chemical and enzymatic treatments, such as urea and β-mercaptoethanol, to facilitate the release, leading to a significant release of proteins (over 40%).
- The findings indicated that the presence of lignocellulose in the cell walls and the low solubility of RuBisCO were major factors preventing its effective release from the TRMCs.
Dynamics of NO Release and Linkage Isomer Formation from S-Nitroso-Mercaptoethanol in Aqueous Solutions: Insights from Femtosecond Infrared Spectroscopy.
J Phys Chem Lett
August 2024
Department of Chemistry and Chemistry Institute for Functional Materials, Pusan National University, Busan 46241, Korea.
Article Synopsis
- Understanding how S-nitroso-thiol (RSNO) acts as a nitric oxide (NO) transporter is crucial for its photochemical uses, with S-nitroso-mercaptoethanol (MceSNO) being a key example due to its water solubility and ability to facilitate quantum calculations.
- Researchers examined the photoexcitation of MceSNO in water, particularly looking at how it breaks down into NO and radicals, recombines, and changes structure, using advanced quantum calculations and femtosecond infrared spectroscopy.
- Results showed that when MceSNO is excited at 320 nm, it quickly breaks apart, with some components recombining into a newly identified structure called Mce
Accurate measurement of sulfhydryls and TCEP-releasable sulfhydryls in the liquid phase of wine that contribute to 'reductive' aromas using LC-MS/MS.
Heliyon
April 2024
The Australian Wine Research Institute, P.O. Box 197, Glen Osmond, SA, 5064, Australia.
Volatile sulfur compounds (VSCs) are important aroma and flavour characters in food and beverage products. The identification and quantification of these extremely reactive and volatile compounds pose analytical challenges which demand selective and sensitive methods. In this study, a novel quantification method was developed to analyse sulfhydryls as well as the total pool of sulfhydryls which can be released after tris(2-carboxyethyl)phosphine (TCEP) addition from disulfides, polysulfides, metal-bound and other yet to be identified sources naturally present in wine.
View Article and Find Full Text PDFEndomorphin-2 analogs with C-terminal esterification display potent antinociceptive effects in the formalin pain test in mice.
Peptides
January 2024
School of Life Science and Technology, Harbin Institute of Technology, 92 West Dazhi Street, Harbin 150001, China; State Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources, Guangxi Normal University, Guilin, China. Electronic address:
Previously, we have investigated three C-terminal esterified endomorphin-2 (EM-2) analogs EM-2-Me, EM-2-Et and EM-2-Bu with methyl, ethyl and tert-butyl ester modifications, respectively. These analogs produced significant antinociception in acute pain at the spinal and supraspinal levels, with reduced tolerance and gastrointestinal side effects. The present study was undertaken to determine the analgesic effects and opioid mechanisms of these three analogs in the formalin pain test.
View Article and Find Full Text PDFPEGylated Polymeric Nanoparticles Loaded with 2-Methoxyestradiol for the Treatment of Uterine Leiomyoma in a Patient-Derived Xenograft Mouse Model.
J Pharm Sci
September 2023
Department of Gynecology & Obstetrics, Johns Hopkins University, Baltimore, MD, USA. Electronic address:
Leiomyomas, the most common benign neoplasms of the female reproductive tract, currently have limited medical treatment options. Drugs targeting estrogen/progesterone signaling are used, but side effects and limited efficacy in many cases are major limitation of their clinical use. Previous studies from our laboratory and others demonstrated that 2-methoxyestradiol (2-ME) is promising treatment for uterine fibroids.
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