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Amplification-free CRISPR/Cas based dual-enzymatic colorimetric nucleic acid biosensing device.
Lab Chip
January 2025
Department of Applied Chemistry, Faculty of Science and Technology, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama 223-8522, Japan.
Nucleic acid testing (NAT) is widely considered the gold standard in analytical fields, with applications spanning environmental monitoring, forensic science and clinical diagnostics, among others. However, its widespread use is often constrained by complicated assay procedures, the need for specialized equipment, and the complexity of reagent handling. In this study, we demonstrate a fully integrated 3D-printed biosensensing device employing a CRISPR/Cas12a-based dual-enzymatic mechanism for highly sensitive and user-friendly nucleic acid detection.
View Article and Find Full Text PDFIt is well known that keratinized mucosa (KM) plays a crucial role for maintaining peri implant health and esthetic outcomes. The Strip Gingival Graft (SGG) technique, which involved an apically positioned flap (APF), in combination with an autogenous SGG and a xenogeneic collagen matrix (XCM), demonstrated its efficacy in re-establishing an adequate amount of KM width at implant sites. Nevertheless, it is still unclear whether harvesting the SGG from the palate (pSGG) or from the buccal aspect of natural dentition (bSGG) affects the esthetic outcomes at the augmented implant sites.
View Article and Find Full Text PDFFluorescent microsphere-based strip for sensitive and quantitative detection of etomidate and metomidate.
Analyst
January 2025
International Joint Research Laboratory for Biointerface and Biodetection, and School of Food Science and Technology, Jiangnan University, Wuxi, People's Republic of China.
In this research, we fabricated a sensitive monoclonal antibody (mAb) 2C3 that targeted etomidate (ET) and metomidate (MT) to establish a lateral-flow immunoassay (LFIA) that incorporated fluorescent microsphere sensors, enabling both the qualitative and quantitative detection of ET and MT within 10 min. Analysis indicated that the visual colorimetric values for ET and MT in water samples were 0.3 μg kg, respectively, with quantitative detection ranges of 0.
View Article and Find Full Text PDFAntifungal, toxicological, and colorimetric properties of Origanum vulgare, Moringa oleifera, and Cinnamomum verum essential oils mixture against Egyptian Prince Yusuf Palace deteriorative fungi.
BMC Biotechnol
January 2025
Faculty of Archaeology, South Valley University, Qena, Egypt.
The increasing demand for sustainable alternatives to conventional antifungal agents has prompted extensive research into the antifungal properties of plant essential oils (EOs). This study investigates the use of EOs mixture (Origanum vulgare, Moringa oleifera, and Cinnamomum verum) for controlling fungal deterioration in wall paintings at the archaeological Youssef Kamal Palace in Nag Hammadi, Egypt. Fungal isolates were collected from deteriorated wall paintings and identified using phenotypic and genotypic analyses.
View Article and Find Full Text PDFChromium Detection in Water Using Optical Methods: A Study of Reagent and Reagentless Approaches.
Crit Rev Anal Chem
January 2025
Department of Electronics and Communication, Vidyavardhaka College of Engineering, Mysuru, India.
Water contaminated with chromium (Cr) poses significant risks to public health and the environment, necessitating reliable detection techniques. This review study uniquely provides a comprehensive analysis of optical methods for detecting Cr pollution in water, focusing on both reagent-based and reagentless approaches, as well as various sensing platforms. Unlike existing reviews that primarily focus on electrochemical and colorimetric/fluorimetric methods, this work highlights the untapped potential of optical technologies, such as colorimetry, SPR, UV-Vis spectroscopy, and more, in detecting distinct Cr species, including reagent and reagentless based approaches.
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