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Ability to produce dormant forms (DF) was demonstrated for non-spore-forming bacteria Staphylococcus aureus (a nonpathogenic strain) and Corynebacterium pseudodiphtheriticum (an organism of the normal oropharyngeal flora). The salient features of the sthaphylococcal and corynebacterial DF were (1) prolonged preservation of viability; (2) resistance to damaging factors (heat treatment); and (3) specific morplology and ultrastructure. The optimal conditions for DF formation were (1) transfer of the stationary-phase cultures into saline solution with CaCl2 (10-300 mM) (for S.
View Article and Find Full Text PDFDifferent capacity for phenotypic variation of Pseudomonas aurantiaca and P. fluorescens, in populations of cyst-like resting cells (CRC), during their germination on solid media, was shown to be a characteristic trait of biodiversity for the dormant forms of these bacteria. This biodiversity manifests itself as qualitative and quantitative differences in the spectra and emergence frequency of phenotype variants, obtained by plating of CRC, and depends on the conditions of CRC formation and storage time.
View Article and Find Full Text PDFCystlike resting cells (CRC) of non-spore-forming gram-negative bacteria of the genus Pseudomonas, P. aurantiaca and P. fluorescens, were obtained and characterized for the first time; their physiological and morphological diversity was demonstrated.
View Article and Find Full Text PDFLymphocyte resistance to cold anabiosis of different degree.
Bull Exp Biol Med
August 2006
Laboratory of Blood Cryophysiology, Institute of Physiology, Komi Research Center, Ural Division of Russian Academy of Sciences, Syktyvkar.
We studied the resistance of human blood T and B lymphocytes to cold anabiosis (-20 degrees C, -40 degrees C, and -80 degrees C) of different duration.
View Article and Find Full Text PDFThe population composition of polycultures of Pseudomonas aeruginosa dissociants (R + M and R + S + M) developing on media with various contents and ratios of nitrogen and phosphorus has been studied. Irrespective of its proportion (10 to 90%) in the inoculum, the R variant accounted for 65 to 84% of the whole population of linear-phase and stationary-phase binary cultures of R and M dissociants, which differ in terms of energy metabolism and nutritional requirements. After prolonged cultivation, the population in the binary culture contained only R cells (100%), which are characterized by minimum requirements with respect to the main biogenic elements.
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