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Qualitative comparison of decalcifiers for mouse bone cryosections for subsequent biophotonic analysis.
Sci Rep
January 2025
Leibniz Institute of Photonic Technology (Member of Leibniz Health Technologies, Member of the Leibniz Centre for Photonics in Infection Research, LPI), 07745, Jena, Germany.
Bone tissue, with its complex structure, often necessitates decalcification of the hard tissue for ex vivo morphological studies. The choice of a suitable decalcification method plays a crucial role in preserving desired features and ensuring compatibility with diverse imaging techniques. The search for a universal decalcification method that is suitable for a range of biophotonic analyses remains an ongoing challenge.
View Article and Find Full Text PDFCraniofacial Effects of Zoledronic Acid on the Osteogenesis Imperfecta Mouse (-/-) Model of Severe Osteogenesis Imperfecta.
Biomedicines
November 2024
Pole of Morphology, Institute of Experimental and Clinical Research, UCLouvain, 1200 Brussels, Belgium.
Osteogenesis imperfecta (OI) is a rare genetic disorder affecting mainly type I collagen, which leads to bone fragility and deformities. OI patients also present craniofacial abnormalities such as macrocephaly and malocclusion. Recently, craniofacial dysmorphism was highlighted in the osteogenesis imperfecta mouse (oim), a validated model of the most severe form of OI.
View Article and Find Full Text PDFOptimizing tissue adherence on glass slides using polyurethane glue: a new slide preparation method.
J Histotechnol
December 2024
Experimental Histopathology Shared Resource, Fred Hutchinson Cancer Center, Seattle, WA, USA.
The application of Clear Gorilla Glue® household adhesive to microscope slides has been found to enhance the mounting and retention of traditionally difficult tissue types, notably bone and tooth specimens. Improvement in end results were observed across H&E staining, immunohistochemistry, and hybridization.
View Article and Find Full Text PDFThe potential calcium content of anchovy on mandibular bone growth through osteoprotegerin expression analysis.
J Oral Maxillofac Pathol
October 2024
Department of Pediatric Dentistry, Faculty of Dentistry, Hasanuddin University, Makassar, Indonesia.
Background: Anchovy is a commonly used food ingredient due to its high calcium content, which supports craniofacial growth. Calcium stimulates the formation of osteoblasts, which produce osteoprotegerin (OPG). OPG binds to RANKL, blocking RANKL-RANK bonding and limiting osteoclast development.
View Article and Find Full Text PDFRapid assessment of the osteogenic capacity of hydroxyapatite/aragonite using a murine tibial periosteal ossification model.
Bioact Mater
March 2025
Centre for Nanohealth, Swansea University Medical School, Faculty of Medicine, Health and Life Science, Swansea University, Swansea, United Kingdom.
Biomaterials are widely used as orthopaedic implants and bone graft substitutes. We aimed to develop a rapid osteogenic assessment method using a murine tibial periosteal ossification model to evaluate the bone formation/remodelling potential of a biomaterial within 2-4 weeks. A novel hydroxyapatite/aragonite (HAA) biomaterial was implanted into C57BL/6 mice juxtaskeletally between the tibia and tibialis anterior muscle.
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