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The treatment of HeLa cells with various concentrations of sodium beta-4-methoxybenzoyl-beta-bromacrylate (Cytembena) results in inhibition of growth and modification of cell cycle distribution. These phenomena were observed at concentrations between 7.5 x 10(-5) and 2.

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The inhibiting effect of Cytembena on HeLa cell kinetics has been demonstrated and analyzed. The percentage of cycling cells decreases, according to the concentration, between 7.5 and 2.

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1. The metabolic disposition of cytembena (sodium cis-3-p-methoxybenzoyl-3-bromoacrylate)labelled at both carbonyl carbon atoms was studied in rats and dogs. 2.

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Renal excretion of Cytembena in subjects with a normal renal function amounted on the average to 7.8% of the given dose during 15 hours following a single intravenous administration of 200 mg of this cytostatic drug. In patients with an impaired renal function there was a further decrease in the urinary Cytembena excretion and this in direct relation to the decrease in endogenous creatinine clearance rate.

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