A method is described for the colonial growth, in semi-solid medium, of erythropoietin-responsive erythroid cell precursors. The erythroid cell precursors were isolated by immune hemolysis from fetal mouse liver. Both the number of precursor cells triggered to proliferate and differentiate, and the size of the erythropoietic colonies formed, are directly dependent upon the concentration of erythropoietin included in the culture.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC388301 | PMC |
| http://dx.doi.org/10.1073/pnas.71.5.1677 | DOI Listing |
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