Acholeplasma axanthum sp. was isolated from the lung of swine with catarrhal pneumonia. Clinical symptoms of respiratory disease, gross and histological lesions of pneumonia, as well as serological response were produced by intranasal inoculation of ;miniature pigs' with the supernatant of lung suspension containing Acholeplasma axanthum and by a 48 hr. broth culture of the strain.A similar picture of disease was observed in animals held in contact with the animals inoculated with untreated lung suspension. Acholeplasma axanthum was isolated from the nasal cavity, lung and peribronchial lymph nodes 7-41 days after inoculation. No lesions were observed after inoculation of pigs with the supernatant of lung suspension pretreated with oxytetracycline or chloroform, and no successful isolation of Acholeplasma axanthum could be achieved after this treatment.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2130498 | PMC |
| http://dx.doi.org/10.1017/s0022172400023500 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
A PCR assay and PCR-restriction fragment length polymorphism combination identifying the 3 primary Mycoplasma species causing mastitis.
J Dairy Sci
January 2012
Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Washington State University, Pullman, WA 99164, USA.
The focus of the current research was to develop real-time PCR assays with improved sensitivity and the capacity to simultaneously speciate the 3 most common mycoplasma mastitis agents: Mycoplasma bovis, Mycoplasma californicum, and Mycoplasma bovigenitalium. Real-time PCR was chosen because it provides rapid results. Partial 16S rRNA gene sequencing was used as the gold standard for evaluating candidate real-time PCR assays.
View Article and Find Full Text PDFAmino acid assimilation by different representatives of Acholeplasma genus has been investigated. It was shown that all 7 investigated typical strains Acholeplasma laidlawii PG-8, A. granularum BTS-39, A.
View Article and Find Full Text PDFAntiserum raised against gyrase A of Acholeplasma laidlawii reacts with phytoplasma proteins.
FEMS Microbiol Lett
January 2002
Faculty of Agriculture, Utsunomiya University, Utsunomiya 321-8505, Japan.
Part of the gyrase A gene (gyrA) of Acholeplasma laidlawii was cloned and incorporated directly downstream from a 6 x His tag segment of the pQE expression vector. The 23-kDa fusion protein was expressed as a 6 x His-tagged protein in Escherichia coli. The fusion protein was purified and used as an antigen for rabbit immunization.
View Article and Find Full Text PDFComparative studies of the persistence of animal mycoplasmas under different environmental conditions.
Vet Microbiol
September 2001
Division of Veterinary Science, Faculty of Agriculture, Miyazaki University, Gakuen-Kibanadai, 889-2192, Miyazaki, Japan.
A comparison of the persistence of mycoplasmas in animals was carried out. When inoculated into liquid media, strains of Mycoplasma bovis, M. arginini, Acholeplasma laidlawii, and A.
View Article and Find Full Text PDFEnzymatic detection of polymerase chain reaction (ED-PCR) was applied for rapid and easy identification of mycoplasmas from contaminated cell culture. This method was based on the capture of amplified products via biotin-streptavidine affinity and the detection of an incorporated hapten in amplified products with enzyme-linked antibody. Primers corresponding to common sequence of Mollicutes in 16S ribosomal RNA dominated gene was used.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!