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Enhanced dextran production by Weissella confusa in co-culture with Candida shehatae and its quorum sensing regulation mechanism.
Int J Biol Macromol
January 2025
Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education & Heilongjiang Provincial Key Laboratory of Plant Genetic Engineering and Biological Fermentation Engineering for Cold Region & Key Laboratory of Microbiology, College of Heilongjiang Province & School of Life Sciences, Heilongjiang University, Harbin 150080, China. Electronic address:
Lactic acid bacteria (LAB) are well-known for its expertise in synthesizing exopolysaccharides (EPS), which are linked to significant health benefits, such as its prebiotic effects and ability to modulate the immune system. However, the synthesis of EPS is hindered by low yields. The objective of this study was to investigate the impact of co-cultivation on EPS output by Weissella confusa XG-3 when paired with Candida shehatae.
View Article and Find Full Text PDFTemperature-regulated cascade reaction for homogeneous oligo-dextran synthesis using a fusion enzyme.
Int J Biol Macromol
October 2024
College of Food and Biological Engineering, Hefei University of Technology, Hefei, China. Electronic address:
Based on the principle of cascade reaction, a fusion enzyme of dextransucrase and dextranase was designed without linker to catalyze the production of oligo-dextran with homogeneous molecular weight from sucrose in one catalytic step. Due to the different effects of temperature on the two components of the fusion enzyme, temperature served as the "toggle switch" for the catalytic efficiency of the two-level fusion enzyme, regulating the catalytic products of the fusion enzyme. Under optimal conditions, the fusion enzyme efficiently utilized 100 % of the sucrose, and the yield of oligo-dextran with a homogeneous molecular weight reached 70 %.
View Article and Find Full Text PDFProteomic and analyses of dextran synthesis influence on AV1n adaptation to temperature change.
Front Microbiol
January 2023
Laboratoire Microorganismes et Biomolécules Actives (LR03ES03), Faculté des Sciences de Tunis, Université Tunis El Manar, Tunis, Tunisia.
is found in vegetables, fruits, and meat and is used by the food industry in the preparation of dairy products, wines, and sugars. We have previously demonstrated that the dextransucrase of (DsrLL) AV1n produces a high-molecular-weight dextran from sucrose, indicating its potential use as a dextran-forming starter culture. We have also shown that this bacterium was able to produce 10-fold higher levels of dextran at 20°C than at 37°C, at the former temperature accompanied by an increase in gene expression.
View Article and Find Full Text PDFBrewers' spent grain as substrate for dextran biosynthesis by Leuconostoc pseudomesenteroides DSM20193 and Weissella confusa A16.
Microb Cell Fact
January 2021
Department of Food and Nutrition, University of Helsinki, 00014, Helsinki, Finland.
Background: Lactic acid bacteria can synthesize dextran and oligosaccharides with different functionality, depending on the strain and fermentation conditions. As natural structure-forming agent, dextran has proven useful as food additive, improving the properties of several raw materials with poor technological quality, such as cereal by-products, fiber-and protein-rich matrices, enabling their use in food applications. In this study, we assessed dextran biosynthesis in situ during fermentation of brewers´ spent grain (BSG), the main by-product of beer brewing industry, with Leuconostoc pseudomesenteroides DSM20193 and Weissella confusa A16.
View Article and Find Full Text PDFDifferent Modes of Regulation of the Expression of Dextransucrase in AV1n and MN1.
Front Microbiol
May 2019
Department of Microbial and Plant Biotechnology, Biological Research Center (CIB), Consejo Superior de Investigaciones Científicas (CSIC), Madrid, Spain.
AV1 strain isolated from a Tunisian avocado was characterized as a dextran producer. The promoter P and the gene encoding the DsrLL dextransucrase responsible for the dextran synthesis were transcriptionally fused to the mCherry coding gene generating the pRCR20 plasmid. Upon plasmid transfer, both AV1n and the dextran non-producing CM70 became red due to expression of the mCherry from the P transcriptional fusion.
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