Coarse powders of acid-insoluble matrix of diaphysis and calvarial parietal bone rapidly and consistently transformed fibroblasts into masses of cartilage and bone containing hemopoietic marrow. The transformant was encapsulated by fibroblasts within 24 hr to form a plaque. Transformation was restricted to the central thicknesses of the plaque. Under the stated conditions the alteration of the phenotype, fibroblast to chondroblast, was an unstable transformation, whereas the phenotype change, fibroblast to osteoblast, was stable. The transformation occurred on a rigid timetable of sequences. Measurements of alkaline phosphatase activity and incorporation of radioactive sulfate, phosphate, and calcium were sensitive and quantitative assays for the appearance of the transformed products, cartilage and bone.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC426757 | PMC |
| http://dx.doi.org/10.1073/pnas.69.6.1601 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Hand Enchondromas Treated with Curettage: a Single Institution Experience and Literature Review.
Acta Chir Orthop Traumatol Cech
January 2025
University of Pisa, Department of Orthopedic and Trauma Surgery, Pisa, Italy.
Purpose Of The Study: Hand enchondromas are benign cartilage bone tumors. Curettage represents the actual gold standard for hand enchondromas. Little has been written about the effectiveness of curettage on hand functionality.
View Article and Find Full Text PDFTreatment of Distal Femoral Malignant Mesenchymal Tumor With Extensor Mechanism Transfer: A Case Report and Surgical Technique.
Cureus
December 2024
Orthopaedics and Traumatology, Cerrahpaşa Faculty of Medicine, Istanbul University-Cerrahpaşa, Istanbul, TUR.
Malignant mesenchymal tumors are a diverse group of aggressive cancers originating from mesenchymal cells in connective tissues such as bone, muscle, cartilage, and fat. These tumors often invade surrounding tissues and metastasize to distant organs, posing significant treatment challenges. Among them, malignant mesenchymal tumors located in the distal femur are particularly rare, with limited reports detailing effective surgical and functional reconstruction strategies following wide resection.
View Article and Find Full Text PDFOsteoperiosteal Cylindrical Iliac Bone Graft and Minced Cartilage as an Osteochondral Autograft for a Large Osteochondral Lesion.
Arthrosc Tech
December 2024
Department of Orthopedics and Traumatology, Ankara Yıldırım Beyazıt University, Ankara Bilkent City Hospital, Ankara, Turkey.
Mosaicplasty is a relatively challenging procedure used in the management of focal osteochondral lesions of the joints. Donor-site morbidity is still the main concern after mosaicplasty because it entails the harvesting of an osteochondral autograft from an otherwise healthy region to be impacted later on the weight-bearing damaged site. We describe a possible alternative to conventional mosaicplasty with subchondral bone support harvested from the iliac crest as an osteoperiosteal autograft and covered with a minced cartilage layer.
View Article and Find Full Text PDFIn Situ Proefferocytosis Microspheres as Macrophage Polarity Converters Accelerate Osteoarthritis Treatment.
Small
January 2025
Department of Orthopedics, Laboratory of Biological Tissue Engineering and Digital Medicine, Affiliated Hospital of North Sichuan Medical College, No. 1 The South of Maoyuan Road, Nanchong, Sichuan, 637000, P. R. China.
Efferocytosis in macrophages typically engages an anti-inflammatory positive feedback regulatory mechanism. In osteoarthritis (OA), characterized by imbalanced inflammatory homeostasis, the proinflammatory state of macrophages in the immune microenvironment can be reversed through enhanced efferocytosis. This study develops an in situ proefferocytosis hydrogel microsphere (macrophage polarity converter, H-C@IL) for OA treatment.
View Article and Find Full Text PDFIdentification of a novel disulfidptosis-related gene signature in osteoarthritis using bioinformatics analysis and experimental validation.
Sci Rep
January 2025
Department of Orthopedic Surgery, 920th Hospital of Joint Logistics Support Force of PLA, Kunming, China.
Osteoarthritis (OA) is a degenerative bone disease characterized by the destruction of joint cartilage and synovial inflammation, involving intricate immune regulation processes. Disulfidptosis, a novel form of programmed cell death, has recently been identified; however, the effects and roles of disulfidptosis-related genes (DR-DEGs) in OA remain unclear. We obtained six OA datasets from the GEO database, using four as training sets and two as validation sets.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!