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Development of a Recombinant Omicron BA.1 Subunit Vaccine Candidate in Pichia pastoris.
Microb Biotechnol
January 2025
Izmir Biomedicine and Genome Center, Izmir, Turkey.
Low-cost and safe vaccines are needed to fill the vaccine inequity gap for future pandemics. Pichia pastoris is an ideal expression system for recombinant protein production due to its cost-effective and easy-to-scale-up process. Here, we developed a next-generation SARS-CoV2 Omicron BA.
View Article and Find Full Text PDFMiddle-throughput LC-MS-based platelet proteomics with minute sample amounts using semi-automated positive pressure FASP in 384-well format (PF384).
Thromb Haemost
January 2025
Department of Bioinformatics, Biocenter, University of Würzburg, Wurzburg, Germany.
Comprehensive characterization of platelets requires various functional assays and analysis techniques, including omics-disciplines, each requiring an individual aliquot of a given sample. Consequently, the sample material per assay is often highly limited rendering downscaling a prerequisite for effective sample exploitation. Here we present a transfer of our recently introduced 96-well-based proteomics workflow (PF96) into the 384-well format (PF384) allowing for a significant increase in sensitivity when processing minute platelet protein amounts.
View Article and Find Full Text PDFScreening and isolation of polyethylene microplastic degrading bacteria from mangrove sediments in southern China.
Sci Total Environ
January 2025
College of Marine Sciences, South China Agricultural University, Guangzhou 510642, China; Zhongshan Innovation Center of South China Agricultural University, Zhongshan 528400, China. Electronic address:
Mangrove sediments in southern China are a large reservoir for microplastics (MPs). In particular, polyethylene microplastics (PE-MPs) are environmentally toxic and have accumulated in large quantities in these sediments, posing a potential threat to the overall mangrove and the organisms that inhabit it. We screened sediments from 5 mangrove sites and identified a potential source of PE-MP degrading bacteria.
View Article and Find Full Text PDFA Dynamic Active Site for OER Catalysis at Nickel-Vanadium-Phosphide Depends on V Surface Position and Fe in Alkaline.
ACS Appl Mater Interfaces
January 2025
Department of Chemistry, American University of Beirut, Beirut, 110236, Lebanon.
Enhancing the rate of the oxygen evolution reaction (OER) by doping Ni-based electrocatalysts with guest metals other than Fe (V in this work) and the stability of the metal site should be assessed independent of Fe traces and in relation to the guest metal activity in solution. We examined OER catalysis and its sustainability at vanadium-doped nickel phosphide (NiP-V) independent of the role of Fe traces in alkaline. V was included in NiP by codeposition at cathodic bias (termed V) or postdeposition during the phosphide-to-hydroxide surface transformation at anodic bias in alkaline spiked with VCl (termed V).
View Article and Find Full Text PDFNisin A Treatment to Protect Honey Bee Larvae from European Foulbrood Disease.
Probiotics Antimicrob Proteins
January 2025
Department of Nutritional Science and Food Safety, Faculty of Applied Bioscience, Tokyo University of Agriculture, Setagaya, Tokyo, 156-8502, Japan.
European foulbrood (EFB) is a bee larvae-specific infectious disease and the causative pathogen is Melissococcus plutonius. Broad-spectrum antibiotics have classically been used in many countries to control the pathogens; however, their use in apiaries was discontinued in several countries due to concerns regarding the health of bees and humans. Therefore, the development of alternative treatments for use in apiaries that are safe for bees and humans is essential.
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