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Genetic Transformation of Somatic Embryos with a Gnk2-like Protein That Reveals a Putative Anti-Oomycete Action.
Plants (Basel)
January 2022
Misión Biológica de Galicia, Consejo Superior de Investigaciones Científicas (MBG-CSIC), Avda Vigo s/n, Campus Vida, Apartado 122, 15705 Santiago de Compostela, Spain.
Holm oak is a key tree species in Mediterranean ecosystems, whose populations have been increasingly threatened by oak decline syndrome, a disease caused by the combined action of and abiotic stresses. The aim of the present study was to produce holm oak plants that overexpress the Ginkbilobin-2 homologous domain gene () that it is known to possess antifungal properties. Proembryogenic masses (PEMs) isolated from four embryogenic lines (Q8, E2, Q10-16 and E00) were used as target explants.
View Article and Find Full Text PDFInduction of hairy roots by various strains of Agrobacterium rhizogenes in different types of Capsicum species explants.
BMC Res Notes
June 2014
Faculty of Applied Sciences, Universiti Teknologi MARA, 40450 Shah Alam, Selangor, Malaysia.
Background: Capsicum annuum and Capsicum frutescens, also known as "chilies", belong to the Solanaceae family and have tremendous beneficial properties. The application of hairy root culture may become an alternative method for future development of these species by adding value, such as by increasing secondary metabolites and improving genetic and biochemical stability compared with normal Capsicum plants. Therefore, in this research, different types of explants of both species were infected with various Agrobacterium rhizogenes strains to provide more information about the morphology and induction efficiency of hairy roots.
View Article and Find Full Text PDFAt a thoroughbred equine breeding farm near Hissar (Haryana), three mares aborted in their seventh month of pregnancy. The vaginal swabs of all aborted mares, and stomach contents, heart blood, liver, spleen and placenta of aborted fetuses yielded pure culture of Aeromonas hydrophila. In addition, A.
View Article and Find Full Text PDFImproved production of transgenic Dioscorea zingiberensis (Dioscoreaceae) by Agrobacterium tumefaciens-mediated transformation.
Genet Mol Res
February 2012
College of Life Science and Technology, Huazhong Agricultural University, Wuhan, Hubei, China.
The establishment of high-efficiency Agrobacterium-mediated transformation techniques could improve the production of Dioscorea zingiberensis, a medicinal species with a high diosgenin content. We co-cultivated embryogenic calli induced from mature seeds with A. tumefaciens strain EHA105.
View Article and Find Full Text PDFA protocol for transformation of Torenia.
Methods Mol Biol
July 2012
National Institute of Floricultural Science, National Agriculture and Food Research Organization, Tsukuba, Ibaraki, Japan.
This chapter describes an Agrobacterium tumefaciens-mediated transformation protocol for torenia, a plant that has several useful characteristics and is primarily used for ornamental and experimental purposes. Leaf segments of torenia were co-cultured with A. tumefaciens containing a vector plasmid for 7 days at 22°C under dark conditions on Murashige and Skoog (MS) medium containing 1 mg/L benzyladenine, 1 mg/L indoleacetic acid, and 100 μM acetosyringone.
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