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Intelligent in-cell electrophysiology: Reconstructing intracellular action potentials using a physics-informed deep learning model trained on nanoelectrode array recordings.
Nat Commun
January 2025
Aiiso Yufeng Li Family Department of Chemical and Nano Engineering, University of California San Diego, La Jolla, CA, USA.
Intracellular electrophysiology is essential in neuroscience, cardiology, and pharmacology for studying cells' electrical properties. Traditional methods like patch-clamp are precise but low-throughput and invasive. Nanoelectrode Arrays (NEAs) offer a promising alternative by enabling simultaneous intracellular and extracellular action potential (iAP and eAP) recordings with high throughput.
View Article and Find Full Text PDFTime-resolved single-cell secretion analysis microfluidics.
Lab Chip
January 2025
Department of Biomedical Engineering, City University of Hong Kong, 83 Tat Chee Avenue, Kowloon Tong, Hong Kong, China.
Revealing how individual cells alter their secretions over time is crucial for understanding their responses to environmental changes. Key questions include: When do cells modify their functions and states? What transitions occur? Insights into the kinetic secretion trajectories of various cell types are essential for unraveling complex biological systems. This review highlights seven microfluidic technologies for time-resolved single-cell secretion analysis: 1.
View Article and Find Full Text PDFSystemically administered platelet-inspired nanoparticles to reduce inflammation surrounding intracortical microelectrodes.
Biomaterials
January 2025
Department of Biomedical Engineering, Case Western Reserve University, Cleveland, OH, United States; Advanced Platform Technology Center, Louis Stokes Cleveland Department of Veterans Affairs Medical Center, Cleveland, OH, United States. Electronic address:
Intracortical microelectrodes (IMEs) are essential for neural signal acquisition in neuroscience and brain-machine interface (BMI) systems, aiding patients with neurological disorders, paralysis, and amputations. However, IMEs often fail to maintain robust signal quality over time, partly due to neuroinflammation caused by vascular damage during insertion. Platelet-inspired nanoparticles (PIN), which possess injury-targeting functions, mimic the adhesion and aggregation of active platelets through conjugated collagen-binding peptides (CBP), von Willebrand Factor-binding peptides (VBP), and fibrinogen-mimetic peptides (FMP).
View Article and Find Full Text PDFThis study characterizes a fluorescent -tdTomato neuronal reporter mouse line with strong labeling of axons throughout the optic nerve, of retinal ganglion cell (RGC) soma in the ganglion cell layer (GCL), and of RGC dendrites in the inner plexiform layer (IPL). The model facilitated assessment of RGC loss in models of degeneration and of RGC detection in mixed neural/glial cultures. The tdTomato signal showed strong overlap with >98% cells immunolabeled with RGC markers RBPMS or BRN3A, consistent with the ubiquitous presence of the vesicular glutamate transporter 2 (VGUT2, SLC17A6) in all RGC subtypes.
View Article and Find Full Text PDFUnveiling the susceptibility of nanosecond pulsed electric field on intracellular function in breast cancerous and normal cells using fluorescence imaging.
Biosens Bioelectron
January 2025
Department of Applied Chemistry and Institute of Molecular Science, National Yang Ming Chiao Tung University, 1001 Ta-Hsueh Rd., Hsinchu, 300093, Taiwan; Center for Emergent Functional Matter Science, National Yang Ming Chiao Tung University, 1001 Ta-Hsueh Rd., Hsinchu, 300093, Taiwan. Electronic address:
Modulation in cellular function and cell death through electrostimulation of intracellular organelles with the application of 50 ns pulsed electric field (nsPEF) have been investigated in breast cancerous MCF7 and normal MCF10A cells by developing a three-dimensional microelectrode device integrated with a fluorescence microscope. The findings revealed that nsPEF induced distinct effects on intracellular functions and dynamics in MCF7 and MCF10A cells. MCF10A cells exhibited significantly higher survivability than MCF7 cells, with different modes of cell death observed between them.
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