In 664 primiparous gilts inseminated twice after oestrus synchronization, the timing of insemination had an important effect on conception rate and litter size. Best results (586 liver piglets per 100 pregnancies) were achieved by a first insemination on the afternoon of the 6th day. Another trial, with sexually mature gilts which were subsequently slaughtered, invloved artificial insemination at a planned time after synchronization of oestus with TRUISYNCHRON plus injection of Prolosan. Pregnancy rate and embryo count on the 29th day after synchronization were good when the first insemination was made on the evening of the 5th day and the second on the evening of the 6th day. Insemination on the morning and evening of the 6th day gave better results than on the evening of the 5th and morning of the 6th day.

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