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Virulence attenuation of Theileria annulata-transformed macrophages.
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Laboratoire de Biologie des Apicomplexes, Faculté de Médecine, Université Paris Descartes - Sorbonne Paris Cité, Paris, France; INSERM U1016, CNRS UMR8104, Cochin Institute, Paris, France. Electronic address:
Tropical theileriosis is a significant tick-borne disease affecting cattle. For decades an empirical live attenuated vaccine has been the primary method of controlling disease. The vaccine is produced through prolonged culture of Theileria annulata schizont-transformed macrophages, but how loss of virulence occurs remains unclear.
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BMC Immunol
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Department of Respiratory And Critical Care Medicine, First Affiliated Hospital of Army Medical University, No. 30 Gaotan Yanzheng Street, Chongqing, 400038, China.
The OP9 culture system is an important in vitro model for B cell development. However, the complex nature of operations and the intrinsic variability of stromal cell functionality, which can be influenced by factors such as radiation exposure or contamination, pose considerable challenges to their wider application. Currently, there exists a paucity of studies documenting in vitro B cell differentiation culture systems that exclude stromal cells, and the experimental methodologies available for reference remain limited.
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The School of Smart Health and Wellness, Zhejiang Dongfang Polytechnic College, Wenzhou, Zhejiang, China.
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Institute of Parasitology, Biomedical Research Center Seltersberg, Justus Liebig University Giessen, Giessen, Germany.
infections have increased in Europe, being more frequent than fasciolosis in some areas. Infection occurs once definitive hosts ingest encysted metacercariae present on vegetation. Following excystation, juvenile flukes penetrate the small intestinal mucosa and migrate into the rumen where adults mature.
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