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Long-term cultivation of retinal pigment epithelium cells on nanofiber scaffolds.
Graefes Arch Clin Exp Ophthalmol
January 2025
Department of Ophthalmology, University Hospital Munster, Munster, Germany.
Purpose: The retinal pigment epithelium (RPE) plays an important role in the pathogenesis of age-related macular degeneration (AMD) and other retinal degenerative diseases. The introduction of healthy RPE cell cultures into the subretinal space offers a potential treatment strategy. The aim of this study was the long-term culture and characterisation of RPE cells on nanofiber scaffolds.
View Article and Find Full Text PDFThe effect of platelet-rich plasma on ferroptosis of nucleus pulposus cells induced by Erastin.
Biochem Biophys Rep
March 2025
Orthopedics of TCM Senior Department, The Sixth Medical Center of PLA General Hospital, Beijing, 100048, China.
Background: Intervertebral disc degeneration (IVDD) has been linked to ferroptosis, a type of programmed cell death. The role of platelet-rich plasma (PRP) in mitigating ferroptosis in nucleus pulposus (NP) cells within IVDD remains unclear.
Purpose: This study aims to verify the effectiveness of PRP in reducing ferroptosis in NP cells induced by Erastin.
Morphometrics and First Ultrastructural Characterizations of Intra-Erythrocytic Stages of So-Called Haemogregarina damiettae Ramadan et al. (1996) With Special Reference to Its Cytopathological Effects on the Infected Erythrocytes.
Microsc Res Tech
January 2025
Department of Zoology and Entomology, Faculty of Science, Helwan University, Helwan, Egypt.
The current study provides the first ultrastructural observations on the intraerythrocytic stages of so-called Haemogregarina damiettae and their cytopathological effects on the infected erythrocytes (IEs) in addition to the recording of new morphometric data. The intraerythrocytic stages are attributed to the immature forms or trophozoites (Ts), and mature gamonts (Gs). Ts are typically bowling-bottle shaped with nucleus (TN) occupying its globose part, while Gs are typically banana- shaped.
View Article and Find Full Text PDFHistochemical analysis of osteoclast and osteoblast distributions on hydroxyapatite/collagen bone-like nanocomposite embedded in rat tibiae.
J Oral Biosci
January 2025
Bioceramics Group, Research Center for Macromoleclules and Biomaterials, National Institute for Materials Science, Tsukuba, Japan. Electronic address:
Objectives: Hydroxyapatite (HAp)/collagen (Col) cylinders with laminated collagen layers were implanted into the tibial diaphysis of rats and examined histochemically to clarify how the orientation of HAp and Col bone-like nanocomposite fibers in HAp/Col blocks affects bone resorption and formation.
Methods: HAp/Col fibers were synthesized and compressed into cylindrical blocks to mimic bone nanostructures. These were implanted into the cortical bone cavities of 10-week-old male Wistar rats with fiber bundles parallel to the tibial surface.
Targeted Activation of OGG1 Inhibits Paraptosis in Lens Epithelial Cells of Early Age-Related Cortical Cataract.
Invest Ophthalmol Vis Sci
January 2025
Eye Institute, Affiliated Hospital of Nantong University, Medical School of Nantong University, Nantong, Jiangsu, China.
Purpose: To investigate potential modes of programmed cell death in the lens epithelial cells (LECs) of patients with early age-related cortical cataract (ARCC) and to explore early-stage intervention strategies.
Methods: Anterior lens capsules were collected from early ARCC patients for comprehensive analysis. Ultrastructural examination of LECs was performed using transmission electron microscopy.
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