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Optimization of FRET imaging in Arabidopsis Protoplasts.
Mol Cells
January 2025
Department of Integrated Biological Science, College of Natural Sciences, Pusan National University, Busan 46241, Republic of Korea; Department of Biological Sciences, College of Natural Sciences, Pusan National University, Busan 46241, Republic of Korea; Institute of Systems Biology, Pusan National University, Busan 46241, Republic Korea. Electronic address:
Recent advancements in fluorescence-based biosensor technologies have enabled more precise and accurate Förster Resonance Energy Transfer (FRET) imaging within Agrobacterium-mediated plant transformation systems. However, the application of FRET imaging in plant tissues remains hindered by significant challenges, particularly the time-intensive process of generating transgenic lines and the complications arising from tissue autofluorescence. In contrast, protoplast-based FRET imaging offers a rapid and efficient platform for functional screening and analysis, making it an essential tool for plant research.
View Article and Find Full Text PDFAntitraction sutures and indocyanine green fluorescence imaging to prevent anastomotic leakage in left-sided colorectal surgery-A video vignette.
Colorectal Dis
January 2025
Department of Colorectal Surgery, Kansai Medical University, Osaka, Japan.
Vulvar and vaginal leiomyosarcomas: Immunohistochemical, molecular genetic and MDM2 fluorescence in situ hybridization analysis of three cases.
Hum Pathol
January 2025
Department of Laboratory Medicine and Pathology, Mayo Clinic, Jacksonville, FL, USA. Electronic address:
Xanthine Nucleosides with Pyrazolo[3,4-]pyrimidine Skeleton: Functionalization with Halogen Atoms, Clickable Side Chains, Pyrene, and iEDDA Cycloadducts, and Impact of Ionic Forms on Photophysical Properties.
J Org Chem
January 2025
Laboratory of Bioorganic Chemistry and Chemical Biology, Center for Nanotechnology, Heisenbergstrasse 11, 48149 Münster, Germany.
Xanthine nucleosides play a significant role in the expansion of the four-letter genetic code. Herein, 7-functionalized 8-aza-7-deazaxanthine ribo- and 2'-deoxyribonucleosides are described. 2-Amino-6-alkoxy nucleosides were converted to halogenated 8-aza-7-deazaxanthine nucleosides by deamination followed by hydroxy/alkoxy substitution.
View Article and Find Full Text PDFFluorescence emission regulation is of great interest for its promising applications in various fields such as microscopy, chemical analysis, encryption, and sensing. Most studies focus on the regulation of the fluorescence emission process. However, the spectral separation of excitation and emission of fluorophores requires careful design of resonances to cover both emission and excitation wavelengths, which is a better choice to enhance fluorescence intensity.
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