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The microcytotoxicity assay and the leukocyte migration inhibition test were unable to reveal any specific reactivity in patients suffering from localized skin melanoma. The non-specific reactivity measured by the microcytotoxicity assay, the so-called NK activity was found to be associated to the cells bearing Fc receptors, irrespective of T and non-T identity. Further more the NK activity depends on the target cell used (established cell lines versus short-term cultures) and the lymphocyte/target cell ratio.

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White blood cells (lymphocytes or leukocytes) obtained from patients suffering from localized skin melanoma and from persons without malignant diseases were tested in the microcytotoxicity assay and leukocyte migration inhibition test using indentical relevant and control targets and antigens. In both test systems the reactivities recorded were found to be independent of target/antigen effector cell combinations, indicating that these interactions were nonspecific. The nonspecific reactivities measured by microcytotoxicity assay (natural cytotoxicity) and the leukocyte migration inhibition test were compared by the simultaneous implementation of the two test systems using white cells from the same blood sample.

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