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Analysis of the Frequency of the A1 and A2 Alleles in the Beta-Casein Gene and the A, B and E Alleles in the Kappa-Casein Gene in Local Cattle Breeds: Polish Red and Polish White-Backed.
Int J Mol Sci
February 2025
Polish Federation of Cattle Breeders and Dairy Farmers in Warsaw, 00-515 Warsaw, Poland.
In view of the threat to local breeds resulting from intensive animal production, many studies are conducted in search of arguments confirming their importance in food production. In the case of milk production, not only is its quantity important, but its quality is as well, including its chemical composition. Particular focus has recently been placed on the casein proteins beta-casein () and kappa-casein (), due to their potential impact on human health or on the suitability of milk for cheese production.
View Article and Find Full Text PDFPredicting Tolerance to Cow's Milk Allergy in Children Using IgE and IgG4 Peptide Binding Profiles.
Cells
February 2025
Servicio de Bioquímica-Investigación, Hospital Universitario Ramón y Cajal, IRYCIS, 28034 Madrid, Spain.
Cow's milk allergy (CMA) is the most common food allergy in infants. This study aimed to identify peptide biomarkers predictive of tolerance in a Spanish population of children with CMA. We investigated specific IgE and IgG4 binding to sequential epitopes of the five major CM allergens (α-s1-, α-s2-, β-, and κ-caseins as well as β-lactoglobulin) using a microarray-based immunoassay.
View Article and Find Full Text PDFA comparative study of the composition of microorganisms and metabolites in different β-casein genetic types of dairy cows based on metagenomics and non-targeted metabolomics.
Food Res Int
March 2025
College of Animal Science and Technology, Ningxia University, Yinchuan 750021, China; Key Laboratory of Ruminant Molecular Cell Breeding in Ningxia, Ningxia University, Yinchuan 750021, China. Electronic address:
β-Casein is the main component of cow's milk protein, with A1 and A2 β-casein being the most common. Of these, A1 β-casein hydrolysate produces BCM-7, which can cause lactose intolerance, while A2 β-casein milk is more gentle on the gut. However, there is limited research on the composition of rumen microbiota, metabolites, and host metabolites in different genotype cows using metagenomics and metabolomics.
View Article and Find Full Text PDFSpecific glycomacropeptide detection via polyacrylamide gel electrophoresis with dual imaging and signal-fusion deep learning.
Food Chem
June 2025
School of Sensing Science and Engineering, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, Shanghai, 200240, China. Electronic address:
Herein, we report a sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) method featuring dual imaging and signal-fusion deep learning for specific identification and analysis of glycomacropeptide (GMP) in milk sample. Conventional SDS-PAGE methods lack specificity because of the signle staining of protein bands, and the overlap between GMP and β-lactoglobulin (βLg). Our dual imaging method generated a pair of complementary detection signals by recruiting intrinsic fluorescence imaging (IFI) and silver staining.
View Article and Find Full Text PDFCasein-based film enriched with lignin as a biodegradable substrate for enzyme immobilization.
RSC Adv
February 2025
Institute of Materials for Electronics and Magnetism, National Research Council (CNR-IMEM) Parco Area delle Scienze, 37/A 43124 Parma Italy
In the last decades, the negative impact of petroleum derived materials on the environment is more and more evident; beyond the unavoidable reduction in the use of classical plastic, another promising approach is the development of alternative materials prepared starting from natural, biodegradable, and more sustainable biomolecules, particularly undervalued or discarded ones. Caseins are the most abundant proteins in milk, with important nutritional value but also interesting film-forming properties. Lignin is a polyphenolic polymer found in wood and derived from a by-product of the cellulose extraction processes; it is well known for its antibacterial, antioxidant, and UV-protecting properties.
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