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Synthesis and characterization of thermally stable and water-soluble pantetheine trisulfide and its composites.
Biosci Biotechnol Biochem
January 2025
Faculty of Pharmacy, Meijo University, 150 Yagotoyama, Tempaku-ku, Nagoya, Japan.
We clarified for the first time the synthesis procedure and characterization of pantetheine trisulfide, a potential new drug. Pantetheine trisulfide is the first supersulfur compound with both thermal stability and water solubility. Its hygroscopicity and deliquescence promote the hydrolysis of trisulfide, but these limitations are overcome by powdered composites of pantetheine trisulfide with silica gel or lactose.
View Article and Find Full Text PDFIsolation and characterization of a β-galactosidase from for industrial processing.
JDS Commun
January 2025
Department of Food Science and Technology, The Ohio State University, Columbus, OH 43210.
In this study, a thermostable β-galactosidase from OSU-PECh-4A has been isolated through diafiltration and size-exclusion chromatography. The enzyme consists of a heterodimer with a molecular mass of 110 kDa, with a small and large subunit of 36 and 74 kDa, respectively. The Michaelis constant (K) and maximum velocity (V) values for lactose and -nitrophenyl-β-d-galactopyranoside (NPG) hydrolysis were, respectively, 29.
View Article and Find Full Text PDFDiscovery and characterization of a new β-glucosidase from Wolfiporia cocos through RNA sequencing and heterologous expression in Escherichia coli.
Int J Biol Macromol
January 2025
The Key Laboratory of Regenerative Biology, Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China.
De novo RNA-sequencing of Wolfiporia cocos mycelia cultured with filter paper composed of cellulose as the sole carbon source revealed a total of five expressed β-glucosidase genes. Among these, the β-glucosidase named Wcbg1B-1, which is composed of 539 amino acid residues and belongs to the GH1 family, had the highest mRNA abundance, accounting for 65 % of the total mRNA of the five expressed β-glucosidases. The recombinant Wcbg1B-1 was successfully expressed in Escherichia coli, with an optimal pH of 6.
View Article and Find Full Text PDFHeterologous Production, Purification and Characterization of Two Cold-Active β-d-Galactosidases with Transglycosylation Activity from the Psychrotolerant Arctic Bacterium sp. S3* Isolated from Spitsbergen Island Soil.
Int J Mol Sci
December 2024
Department of Pharmaceutical Technology and Biochemistry, Faculty of Chemistry, Gdansk University of Technology, Narutowicza 11/12, 80-233 Gdansk, Poland.
Cold-adapted microorganisms possess cold-active enzymes with potential applications in different industries and research areas. In this study, two genes encoding β-d-galactosidases belonging to Glycoside Hydrolase families 2 and 42 from the psychrotolerant Arctic bacterium sp. S3* were cloned, expressed in and , purified and characterized.
View Article and Find Full Text PDFEngineering an Escherichia coli surface display platform based on an autotransporter from Stenotrophomonas maltophilia: Autodisplay of enzymes with low to high molecular weight.
J Biotechnol
February 2025
State Key Laboratory of Marine Food Processing and Safety Control, College of Food Science and Engineering, Ocean University of China, Qingdao 266404, PR China; Qingdao Key Laboratory of Food Biotechnology, Qingdao 266404, PR China; Key Laboratory of Biological Processing of Aquatic Products, China National Light Industry, Qingdao 266404, PR China. Electronic address:
Surface display technology has garnered significant attention for preparing efficient whole cell catalysts, while reported carrier proteins still cannot meet the demand to display various passenger domains, especially for those with high molecular weight. This study demonstrates that the autotransporter of esterase Est7 (E7AT) from Stenotrophomonas maltophilia played a decisive role in its efficient surface display. Guided by the original signal peptide, the surface display ratio of Est7 was determined as 89.
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