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Temperature-driven CsgA-enhanced mutant enable distinct self-assembly.
Int J Biol Macromol
January 2025
State Key Laboratory of Materials-Oriented Chemical Engineering, College of Food Science and Light Industry, Nanjing Tech University, Nanjing 211816, China. Electronic address:
Functional coating materials have found extensive applications across various technological fields. However, the effectiveness of these coating depends critically on the choice of an appropriate medium. In this study, we developed an advanced "molecular glue", a CsgA variant known as CsgA-pro, which can serve as a versatile medium for biotherapy.
View Article and Find Full Text PDFThe chloroplast RNA-binding protein CP29A supports expression during cold acclimation.
Proc Natl Acad Sci U S A
February 2025
Molecular Genetics, Institute of Biology, Faculty of Life Sciences, Humboldt Universität zu Berlin, Berlin 10115, Germany.
The chloroplast genome encodes key components of the photosynthetic light reaction machinery as well as the large subunit of the enzyme central for carbon fixation, Ribulose-1,5-bisphosphat-carboxylase/-oxygenase (RuBisCo). Its expression is predominantly regulated posttranscriptionally, with nuclear-encoded RNA-binding proteins (RBPs) playing a key role. Mutants of chloroplast gene expression factors often exhibit impaired chloroplast biogenesis, especially in cold conditions.
View Article and Find Full Text PDFStructured Liquid--Liquid Emulsion Stabilized by Surface-Engineered Liquid Metal Droplets as "Mutant" Pickering Emulsifiers.
ACS Appl Mater Interfaces
January 2025
Department of Chemical Engineering, Myongji University, Yongin 17058, Republic of Korea.
Liquid metals (LMs), i.e., metals and alloys that exist in a liquid state at room temperature, have recently attracted considerable attention owing to their electronic and rheological properties useful in various cutting-edge technologies.
View Article and Find Full Text PDFRice glycosyltransferase OsDUGT1 is involved in heat stress tolerance by glycosylating flavonoids and regulating flavonoid metabolism.
Front Plant Sci
January 2025
The Key Laboratory of Plant Development and Environmental Adaptation Biology, Ministry of Education, Shandong Key Laboratory of Precision Molecular Crop Design and Breeding, School of Life Sciences, Shandong University, Qingdao, China.
One significant environmental element influencing the growth and yield of rice ( L.) is high temperature. Nevertheless, the mechanism by which rice responds to high temperature is not fully understood.
View Article and Find Full Text PDFModelling the human Coenzyme Q deficiency in Drosophila melanogaster.
Free Radic Biol Med
January 2025
Centro Andaluz de Biología del Desarrollo, Universidad Pablo de Olavide-CSIC-JA, Sevilla, Spain; CIBERER, U729, Instituto de Salud Carlos III, Madrid, Spain. Electronic address:
The interference of the expression of each of the genes involved in the synthesis of coenzyme Q (CoQ) in Drosophila melanogaster can help to understand the pathophysiology of CoQ-dependent mitochondrial diseases in humans. We have knocked-down all genes involved in the CoQ biosynthesis pathway at different temperatures to induce depletion of CoQ at different levels throughout the body and in a tissue-specific manner. The efficiency of the knockdowns was quantified by Q-RTPCR and determination of CoQ levels by HPLC-UV+ECD.
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