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Protein Structural Modeling and Transport Thermodynamics Reveal That Plant Cation-Chloride Cotransporters Mediate Potassium-Chloride Symport.
Int J Mol Sci
December 2024
School of Agriculture, Food and Wine, Waite Research Institute, Faculty of Sciences, Engineering and Technology, University of Adelaide, Waite Campus Precinct, Glen Osmond, Adelaide, SA 5064, Australia.
Plant cation-chloride cotransporters (CCCs) are proposed to be Na-K-2Cl transporting membrane proteins, although evolutionarily, they associate more closely with K-Cl cotransporters (KCCs). Here, we investigated grapevine ( L.) VvCCC using 3D protein modeling, bioinformatics, and electrophysiology with a heterologously expressed protein.
View Article and Find Full Text PDFShort life fission products extracted from molten salt reactor fuel for radiopharmaceutical applications.
Appl Radiat Isot
March 2024
School of Engineering, Lancaster University, Lancaster LA1 4YW, UK.
This work studies the potential of using short life fission product (Fp) radioisotopes e.g. Br, Rb, (Sr) - Y, (Mo) - Tc, Ru - Rh, Ag, Sb - Te, I, I, Xe, Cs, Ce, Ce, Pr, Nd - Pm, Pm, Sm, Eu, Gd and Tb, extracted from a molten salt reactor and their separation using specific thermodynamic and radiochemical conditions.
View Article and Find Full Text PDFA Na pump with reduced stoichiometry is up-regulated by brine shrimp in extreme salinities.
Proc Natl Acad Sci U S A
December 2023
School of Biological Sciences, Illinois State University, Normal, IL 61790.
Brine shrimp () are the only animals to thrive at sodium concentrations above 4 M. Salt excretion is powered by the Na,K-ATPase (NKA), a heterodimeric (αβ) pump that usually exports 3Na in exchange for 2 K per hydrolyzed ATP. express several NKA catalytic α-subunit subtypes.
View Article and Find Full Text PDFDietary Na depletion up-regulates NKCC1 expression and enhances electrogenic Cl secretion in rat proximal colon.
Cell Mol Life Sci
July 2023
Departments of Biochemistry and Molecular Medicine, West Virginia University School of Medicine, 1 Medical Center Drive, Morgantown, WV, 26506, USA.
The corticosteroid hormone, aldosterone, markedly enhances K secretion throughout the colon, a mechanism critical to its role in maintaining overall K balance. Previous studies demonstrated that basolateral NKCC1 was up-regulated by aldosterone in the distal colon specifically to support K secretion-which is distinct from the more well-established role of NKCC1 in supporting luminal Cl secretion. However, considerable segmental variability exists between proximal and distal colonic ion transport processes, especially concerning their regulation by aldosterone.
View Article and Find Full Text PDFHigh-throughput cell-based assays for identifying antagonists of multiple smoking-associated human nicotinic acetylcholine receptor subtypes.
SLAS Discov
January 2022
Division of Neurobiology, Barrow Neurological Institute, St. Joseph's Hospital and Medical Center, 350 W. Thomas Rd., Phoenix, AZ 85013, United States. Electronic address:
There is substantial evidence that in addition to nicotine, other compounds found in tobacco smoke significantly influence smoking behavior. Further, recent years have seen an explosion in the availability of non-combusted products that deliver nicotine, such as e-cigarettes and "home-brew" vaping devices that are essentially unregulated. There are many thousands of compounds in tobacco smoke alone, and new products are constantly introducing new compounds.
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