Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Fluorometric enzymatic assay of l-arginine.
Spectrochim Acta A Mol Biomol Spectrosc
January 2017
Institute of Cell Biology, National Academy of Science of Ukraine, Drahomanov Str. 14/16, 79005 Lviv, Ukraine.
The enzymes of l-arginine (further - Arg) metabolism are promising tools for elaboration of selective methods for quantitative Arg analysis. In our study we propose an enzymatic method for Arg assay based on fluorometric monitoring of ammonia, a final product of Arg splitting by human liver arginase I (further - arginase), isolated from the recombinant yeast strain, and commercial urease. The selective analysis of ammonia (at 415nm under excitation at 360nm) is based on reaction with o-phthalaldehyde (OPA) in the presence of sulfite in alkali medium: these conditions permit to avoid the reaction of OPA with any amino acid.
View Article and Find Full Text PDFRapid assays for Gaucher and Hurler diseases in dried blood spots using digital microfluidics.
Mol Genet Metab
June 2013
Advanced Liquid Logic, Inc., Research Triangle Park, NC 27709, USA.
Objective: Easy tool for newborn screening of Gaucher and Hurler diseases.
Methods: Method comparison between fluorometric enzymatic activity assay on a digital microfluidic platform and micro-titer plate bench assay was performed on normal (n = 100), Gaucher (n = 10) and Hurler (n = 7) dried blood spot samples.
Results: Enzymatic activity analysis of glucocerebrosidase (Gaucher) and α-l-iduronidase (Hurler) revealed similar discrimination between normal and affected samples on both platforms.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!