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A preliminary evaluation of a fast, low-cost, and high-throughput nucleic acid extraction method for bacterial microbiota profiling in low-microbial biomass samples.
Sci Rep
January 2025
Department of Microbiology, Division of Laboratory Medicine, Oslo University Hospital, 0372, Oslo, Norway.
The respiratory tract is colonized with low-density microbial communities, which have been shown to impact human respiratory health through microbiota-host interactions. However, a lack of fast and cost-effective nucleic acid extraction method for low-microbial biomass samples hinders investigation of respiratory microbiota. Here, we performed a pilot study to assess the suitability of the NAxtra nucleic acid extraction protocol for profiling bacterial microbiota in respiratory samples.
View Article and Find Full Text PDFDetermining the Effect of Varied Proportions of Cohort Administered Tulathromycin at Arrival on Nasopharyngeal Microbiota and Performance Characteristics in Yearling Steers in the First 56 Days on Feed.
Microorganisms
December 2024
Beef Cattle Institute, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506, USA.
Metaphylaxis or treating the entire population of cattle at arrival with an antimicrobial has been studied extensively in the cattle industry; however, little information is available on the impacts of treating only a proportion of the population with antimicrobials at arrival. The study objective was to determine potential associations between the proportion of animals in a pen treated with antimicrobial therapy with pen performance and nasopharyngeal microbiome. Yearling steers (n = 160) were randomly allocated to study pens (n = 40) and pens were systematically randomized to one of two antimicrobial treatments (META: all four head received tulathromycin; MIXED: two of four head randomly selected to receive tulathromycin).
View Article and Find Full Text PDF[Exploring local microbial communities in adenoids through 16S rRNA gene sequencing].
Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi
January 2025
To explore the hypothesis of "pathogen storage pool" by analyzing the local microbial community of adenoids. Under the guidance of a 70° nasal endoscope, sterile swabs were used to collect secretions from the adenoid crypts of the subjects. The samples were sent to the laboratory for DNA extraction and standard bacterial 16S full-length sequencing analysis.
View Article and Find Full Text PDFSpatiotemporal dynamics of the oropharyngeal microbiome in a cohort of Ivorian school children.
Sci Rep
December 2024
Centre Suisse de Recherches Scientifiques en Côte d'Ivoire (CSRS), Abidjan, Côte d'Ivoire.
The respiratory tract harbours microorganisms of the normal host microbiota which are also capable of causing invasive disease. Among these, Neisseria meningitidis a commensal bacterium of the oropharynx can cause meningitis, a disease with epidemic potential. The oral microbiome plays a crucial role in maintaining respiratory health.
View Article and Find Full Text PDFMicrobial Dysbiosis in Nasopharyngeal Carcinoma: A Pilot Study on Biomarker Potential.
J Otolaryngol Head Neck Surg
December 2024
Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan.
Importance: Nasopharyngeal carcinoma (NPC) is closely linked to microorganisms, especially intra-tumoral microbiota. However, the role of commensal microbiota in NPC remains underexplored, with implications for understanding disease mechanisms.
Objective: This study aims to analyze and compare the bacterial microbiota in the nasopharynx and middle meatus (MM) of individuals with NPC and those without NPC.
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