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Genome stability of the vaccine strain VACΔ6.
Vavilovskii Zhurnal Genet Selektsii
July 2022
State Research Center of Virology and Biotechnology "Vector", Rospotrebnadzor, Koltsovo, Novosibirsk region, Russia.
Article Synopsis
- The decline in mass smallpox vaccination since 1980 has led to a significant reduction in human immunity against orthopoxvirus infections, raising the risks of zoonotic diseases like monkeypox and cowpox.
- Traditional smallpox vaccines are not suitable for mass use due to their reactogenicity, prompting the need for modern live vaccines using genetic engineering techniques.
- Researchers developed the VACΔ6 strain by deleting virulence genes and conducting extensive passaging, ensuring minimal off-target mutations, and performing genomic stability tests to confirm its potential as a safe vaccine candidate.
A high cell density perfusion process for Modified Vaccinia virus Ankara production: Process integration with inline DNA digestion and cost analysis.
Biotechnol Bioeng
December 2021
Bioprocess Engineering Group, Max Planck Institute for Dynamics of Complex Technical Systems, Magdeburg, Germany.
By integrating continuous cell cultures with continuous purification methods, process yields and product quality attributes have been improved over the last 10 years for recombinant protein production. However, for the production of viral vectors such as Modified Vaccinia virus Ankara (MVA), no such studies have been reported although there is an increasing need to meet the requirements for a rising number of clinical trials against infectious or neoplastic diseases. Here, we present for the first time a scalable suspension cell (AGE1.
View Article and Find Full Text PDFEthical Challenges Using Human Tumor Cell Lines in Cancer Research.
Recent Results Cancer Res
May 2021
Leibniz Institute DSMZ, German Collection of Microorganisms and Cell Cultures GmbH, Inhoffenstraße 7 B, 38124, Braunschweig, Germany.
Human and animal cell cultures are indispensable model systems for the biomedical research and pharmaceutical industry and already represent one of the most important alternatives to animal experiments. The development of mammalian cell culture started in the first half of the last century when fundamental questions of genetics were unresolved and the pioneers of cell culture did not care about individual personality rights of donors of biomaterials. However, cultivation of primary and continuous cell cultures was and still is usually associated with the use of FBS, which-almost universally applicable-is questionable in terms of extraction and quality variations measurably affecting reproducibility of results.
View Article and Find Full Text PDFProduction of Modified Vaccinia Ankara Virus by Intensified Cell Cultures: A Comparison of Platform Technologies for Viral Vector Production.
Biotechnol J
January 2021
Max Planck Institute for Dynamics of Complex Technical Systems, Bioprocess Engineering, Sandtorstr. 1, Magdeburg, 39106, Germany.
Modified Vaccinia Ankara (MVA) virus is a promising vector for vaccination against various challenging pathogens or the treatment of some types of cancers, requiring a high amount of virions per dose for vaccination and gene therapy. Upstream process intensification combining perfusion technologies, the avian suspension cell line AGE1.CR.
View Article and Find Full Text PDFCombination therapy with oncolytic viruses and immune checkpoint inhibitors.
Expert Opin Biol Ther
June 2020
Division of Radiotherapy and Imaging, The Institute of Cancer Research, London, UK.
: Immune checkpoint inhibitors (ICI) have dramatically improved the outcome for cancer patients across multiple tumor types. However the response rates to ICI monotherapy remain relatively low, in part due to some tumors cultivating an inherently 'cold' immune microenvironment. Oncolytic viruses (OV) have the capability to promote a 'hotter' immune microenvironment which can improve the efficacy of ICI.
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