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Characterization by LC-MS/MS analysis of KLH vaccine conjugated with a tick antigen peptide.
Analyst
January 2025
Department of Proteomics, Mass Spectrometry Laboratory, Center for Genetic Engineering and Biotechnology, 31 Avenue, Cubanacan, Playa, Havana, Cuba.
Keyhole limpet haemocyanins (KLH1 and KLH2) from , are multi-subunit oxygen-carrying metalloproteins of approximately 3900 amino acids, that are widely used as carrier proteins in conjugate vaccines and in immunotherapy. KLHs and their derived conjugate vaccines are poorly characterized by LC-MS/MS due to their very stable supramolecular structures with megadalton molecular mass, and their resistance to efficient digestion with standard protocols. KLH1 and KLH2 proteins were conjugated to the conserved P0 peptide (pP0), derived from the P0 acidic ribosomal protein of sp.
View Article and Find Full Text PDFComparison of data augmentation and classification algorithms based on plastic spectroscopy.
Anal Methods
January 2025
Jiangsu Beier Machinery Co. Ltd, Jiangsu, 215600, China.
Plastic waste management is one of the key issues in global environmental protection. Integrating spectroscopy acquisition devices with deep learning algorithms has emerged as an effective method for rapid plastic classification. However, the challenges in collecting plastic samples and spectroscopy data have resulted in a limited number of data samples and an incomplete comparison of relevant classification algorithms.
View Article and Find Full Text PDFExploring the Key Indicators for Off-Flavor in Industrial Osmanthus Absolute through Sensomics and Chemometric Approaches.
J Agric Food Chem
January 2025
Zhengzhou Tobacco Research Institute of CNTC, Fengyang Street #2, Zhengzhou, Henan 450001, PR China.
The occurrence of off-flavor in osmanthus absolutes has emerged as a significant concern that could hinder its broad market acceptance and associated economic development. In this study, key off-flavor molecules in industrial osmanthus absolute were identified through sensomics and chemometric approaches. A group of 10 off-flavor (OF) samples, eliciting smoky/phenolic, sweaty/sour, and spicy odors, were compared with 10 pleasant aroma (PA) samples through various analyses, including overall aroma assessment, comprehensive chemical profiling, aroma extract dilution analysis (AEDA), and orthogonal partial least-squares-discriminant analysis (OPLS-DA).
View Article and Find Full Text PDFReasons for retraction of clinical research articles in PubMed indexed medical journals from 2012 to 2022.
Indian J Med Ethics
January 2025
Department of Clinical Pharmacology, Toipwala National Medical College and BYL Nair Charitable Hospital, Mumbai 400008, Maharashtra, INDIA.
Background: Misconduct in the publication of research articles is a serious concern for the scientific community. This study was conducted with the objective to assess various reasons for retraction of clinical research articles published in PubMed indexed journals from all over the world since 2012 to 2022.
Methods: A search was performed on the PubMed database for retracted research articles using filters for "retracted publication".
Antifouling Copper Surfaces Interfere with Wet Chemical Nitrate Sensors: Characterization and Mechanistic Investigation.
ACS ES T Water
January 2025
Mechanical Engineering, Faculty of Engineering and Physical Sciences, University of Southampton, Southampton, SO17 1BJ, United Kingdom.
Wet chemical sensors autonomously sample and analyze water using chemical assays. Their internal fluidics are not susceptible to biofouling (the undesirable accumulation of microorganisms, algae, and animals in natural waters) due to the harsh chemical environment and dark conditions; however, the sample intake and filter are potentially susceptible. This paper describes the use of copper intake filters, incorporated to prevent fouling, on two different wet chemical nitrate sensors that each use different variants of the Griess assay (in particular, different nitrate reduction steps) to quantify nitrate concentrations.
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