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Ultrasensitive "On-Off" Ratiometric Fluorescence Biosensor Based on RPA-CRISPR/Cas12a for Detection of .
J Agric Food Chem
January 2025
School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
() is a major pathogenic bacterium responsible for bacterial foodborne diseases, making its rapid, specific, and accurate detection crucial. In this study, we develop a ratiometric biosensor based on the recombinase polymerase amplification-clustered regularly interspaced short palindromic repeats/CRISPR associated protein 12a (RPA-CRISPR/Cas12a) system and Eu-metal-organic framework (Eu-MOF) fluorescent nanomaterials for the high-sensitivity detection of , combining with RPA for efficient isothermal amplification, this sensor enhances specificity and sensitivity by utilizing the target activation of CRISPR/Cas12a. The Eu-MOF serves a dual function, providing stable red fluorescence as a reference signal and adsorbing FAM-labeled probes for fluorescence quenching, forming a dual-signal system that significantly reduces background interference.
View Article and Find Full Text PDFThe impact of spray-induced gene silencing on cereal phyllosphere microbiota.
Environ Microbiome
January 2025
Department of Plant Breeding, Swedish University of Agricultural Sciences, Alnarp, Sweden.
Background: Fusarium head blight (FHB) is a major disease affecting cereal crops including wheat, barley, rye, oats and maize. Its predominant causal agent is the ascomycete fungus Fusarium graminearum, which infects the spikes and thereby reduces grain yield and quality. The frequency and severity of FHB epidemics has increased in recent years, threatening global food security.
View Article and Find Full Text PDFAntimicrobial Activity of Compounds Isolated from the Nest Material of (Mayr) (Hymenoptera: Formicidae).
Insects
December 2024
College of Plant Protection, South China Agricultural University, Guangzhou 510642, China.
Ants as social insects live in groups, which increases the risk of contagious diseases. In response to the threat of pathogens, ants have evolved a variety of defense mechanisms, including incorporating antimicrobial chemicals into nest material for nest hygiene. is an arboreal ant, building its nest using plant tissues.
View Article and Find Full Text PDFComparative Genomic Analysis of BRI3 Reveals Genes Potentially Associated with Efficient Antagonism of (Lib.) de Bary.
Genes (Basel)
December 2024
National Key Laboratory of Agricultural Microbiology, Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Background/objectives: has recently received increased attention as a potential biological agent because of its broad-spectrum antagonistic capacity against harmful bacteria and fungi. This study aims to thoroughly analyze the genomic characteristics of BRI3, thereby providing theoretical groundwork for the agronomic utilization of this strain.
Methods: In this work, we evaluated the beneficial traits of the newly isolated strain BRI3 via in vitro experiments, whole-genome sequencing, functional annotation, and comparative genomic analysis.
Electro-polymerization of modified carbon paste sensor for detecting azithromycin.
Sci Rep
January 2025
Chemistry Department, Faculty of Science, Cairo University, Giza, 12613, Egypt.
Azithromycin (AM) is one of the prescribed drugs in pandemic medication treatment which has paid great attention. We developed in this study a simply modified carbon paste electrode (CPE) to detect AM using poly-threonine (PT). PT or similar polymers are used as carriers to enhance the delivery and effectiveness of AM.
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