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β-ecdysone/PLGA composite scaffolds promote skull defect healing in diabetic rat.
Front Bioeng Biotechnol
January 2025
Guangxi Key Laboratory of Oral and Maxillofacial Rehabilitation and Reconstruction, College and Hospital of Stomatology, Guangxi Medical University, Nanning, Guangxi, China.
Introduction: Diabetes mellitus often leads to bone metabolism disorders, hindering bone regeneration and delaying the healing of bone defects. β-Ecdysone, a plant-derived hormone known for its wide range of physiological activities, possesses hypoglycemic effects and promotes osteogenic differentiation. This study developed a composite PLGA slow-release scaffold loaded with β-ecdysone to enhance its bioavailability through topical administration and to investigate its potential to heal diabetic bone defects.
View Article and Find Full Text PDFEvaluation of a Simple Antibiotic-Free Cryopreservation Protocol for Drone Semen.
Insects
January 2025
Fundamental and Applied Research for Animals and Health Research Unit (FARAH), Comparative Veterinary Medicine, Faculty of Veterinary Medicine, University of Liège, 4000 Liège, Belgium.
The increasing reliance of modern agriculture on honey bee () pollination has driven efforts to preserve and enhance bee populations. The cryopreservation of drone semen presents a promising solution for preserving genetic diversity and supporting breeding programs without live animal transport risks. This study aimed to evaluate a one-step dilution antibiotic-free drone semen slow-freezing protocol under field conditions with in vitro and in vivo parameters.
View Article and Find Full Text PDFCarboxymethyl freeze-thawed tapioca starch moderates oil deterioration in deep-frying cycles of battered sausages.
Food Chem
January 2025
College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, China; National Engineering Research Center for Wheat and Corn Deep Processing, Jilin Agricultural University, Changchun 130118, China. Electronic address:
This study was aimed at investigating the effect of carboxymethyl freeze-thawed tapioca starch (CM-FTS) in the batter wrapped ham sausages (CM-FTS-BHS) on the oil qualities following deep-frying cycles compared with tapioca starch, freeze-thawed tapioca starch, and carboxymethyl tapioca starch. As the deep-frying cycles increased, the degree of all oil deterioration continued to increase. Analysis of oxidation, acid values, carbonyl values, conjugated diene/triene content and low-field magnetic resonance revealed that adding CM-FTS to batter was more conducive to reducing the formation of oil deterioration products than other samples.
View Article and Find Full Text PDFEpididymal bull sperm selection by Percoll® density-gradient centrifugation prior to conventional or ultra-rapid freezing enhances post-thaw sperm quality.
Cryobiology
January 2025
Laboratorio de Biotecnología de la Reproducción Animal, Facultad de Ciencias Agropecuarias, Universidad de Cuenca, EC010205, Cuenca, Ecuador. Electronic address:
This study evaluated the effectiveness of Percoll® density gradient centrifugation (Percoll-DGC) for selecting bull epididymal sperm prior to conventional slow (CS) or ultra-rapid (UR) freezing and its effects on sperm quality. Fifteen pooled samples from 30 epididymides (2 different samples/pool) of 15 bulls were split into two aliquots assigned to either CS or UR freezing. Samples were either selected using Percoll-DGC (40/80 %) or left non-selected (control), resulting in four pre-freezing treatments: Percoll-CS, Control-CS, Percoll-UR, and Control-UR.
View Article and Find Full Text PDFQuick Freezing-Induced Au Nanoparticle Aggregates (QFIAAs) for Near-IR (NIR) Surface-Enhanced Raman Scattering (SERS) Substrates.
Langmuir
January 2025
Department of Chemistry, SUNY Buffalo State University, 1300 Elmwood Ave., Buffalo, New York 14222, United States.
Here, we report a simple method to prepare near-IR (NIR) surface-enhanced Raman scattering (SERS) substrates by quickly freezing a citrate-capped Au nanoparticle (AuNP) solution in liquid nitrogen, followed by thawing it at room temperature. This process aggregates AuNPs in a controlled manner by forming ice crystals with smaller grain sizes when compared to a slow freezing process. The resulting smaller AuNP aggregates remain suspended in solution long enough to conduct high-throughput chemical analysis in a microwell plate using the NIR SERS spectroscopy.
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