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One-pot ligation of multiple mRNA fragments on dsDNA splint advancing regional modification and translation.

Nucleic Acids Res

January 2025

Key Laboratory of Bio-resource and Eco-environment of Ministry of Education, The College of Life Sciences, Sichuan University, 24 South Section 1, 1st Ring Road, Chengdu, Sichuan 610064, P.R. China.

Region-specific RNA modifications are crucial for advancing RNA research and therapeutics, including messenger RNA (mRNA)-based vaccines and immunotherapy. However, the predominant method, synthesizing regionally modified mRNAs with short single-stranded DNA (ssDNA) splints, encounters challenges in ligating long mRNA fragments due to the formation of RNA self-folded complex structures. To address this issue, we developed an efficient strategy using an easily obtained long double-stranded DNA (dsDNA) as a ligation splint after in situ denaturing, while parts of this dsDNA are the templates for transcribing mRNA fragments.

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Modification of gemcitabine with oxaliplatin in China for unresectable gallbladder cancer: a cost-effectiveness analysis.

Front Public Health

December 2024

Department of Pharmacy, National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, Chengdu, Sichuan, China.

Background: The incidence of gall bladder cancer (GBC), one of the most prevalent bile duct malignancies, differs with ethnicity and geographic location. To treat unresected GBC in the Chinese setting, this study aimed to assess the financial effectiveness of a combination of modified gemcitabine and oxaliplatin.

Methods: Data from a randomized controlled study in which individuals with metastatic GBC were treated with oxaliplatin and gemcitabine demonstrated improved survival.

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Cytidine analogs in plant epigenetic research and beyond.

J Exp Bot

December 2024

Centre of Plant Structural and Functional Genomics, Institute of Experimental Botany, Czech Acad Sci, Šlechtitelů 31, Olomouc 77900, Czech Republic.

Cytosine (DNA) methylation plays important roles in silencing transposable elements, plant development, genomic imprinting, stress responses, and maintenance of genome stability. To better understand the functions of this epigenetic modification, several tools have been developed to manipulate DNA methylation levels. These include mutants of DNA methylation writers and readers, targeted manipulation of locus-specific methylation, and the use of chemical inhibitors.

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Background: Recently, the incidence of pancreatic cancer (PC) has gradually increased. Research has shown that UTX mutants are critical in tumors. However, the underlying mechanisms remain incompletely understood.

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Compression force promotes the osteogenic differentiation of periodontal ligament stem cells by regulating NAT10-mediated ac4C modification of BMP2.

J Orthop Surg Res

December 2024

Department of Oral Orthodontics, The First Affiliated Hospital, Zhengzhou University, Jianshe East Road, Erqi District, Zhengzhou, 450052, Henan, China.

Background: Orthodontic treatment applies specific corrective forces to teeth, transmitting stress to periodontal tissue, thereby regulating the growth and development of periodontal ligament stem cells (PDLSCs). Recently, N-acetyltransferase 10 (NAT10) mediated N4-acetylcytidine (ac4C) modification is demonstrated to play a key role in the osteogenic differentiation of stem cells. Therefore, this study aimed explore the effects of Orthodontic treatment on the NAT10 mediated ac4C modification and osteogenic differentiation of PDLSCs.

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