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A simple and efficient TALEN system for genome editing in plants.
Plant Mol Biol
January 2025
Key Laboratory of Genetics, Breeding and Multiple Utilization of Crops, Ministry of Education, Key Laboratory of Biological Breeding for Fujian and Taiwan Crops, Ministry of Agriculture and Rural Affairs, Key Laboratory of Crop Biotechnology of Fujian Higher Education Institutes, College of Agriculture, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
Design and Characterization of a Gene-Encoding DNA Nanoparticle in a Cell-Free Transcription-Translation System.
ACS Appl Nano Mater
June 2024
Department of Chemistry, College of Arts and Sciences, Case Western Reserve University, Cleveland, Ohio 44106, United States.
DNA nanotechnology has made initial progress toward developing gene-encoded DNA origami nanoparticles (NPs) that display potential utility for future gene therapy applications. However, due to the challenges involved with gene delivery into cells including transport through the membrane, intracellular targeting, and inherent expression of nucleases along with interference from other active proteins, it can be difficult to more directly study the effect of DNA NP design on subsequent gene expression. In this work, we demonstrate an approach for studying the expression of gene-encoding DNA origami NPs without the use of cells.
View Article and Find Full Text PDFBacteria are constantly threatened by their viral predators (phages), which has resulted in the development of defense systems for bacterial survival. One family of defense systems found widely across bacteria are OLD (for overcoming lysogeny defect) family nucleases. Despite recent discoveries regarding Class 2 and 4 OLD family nucleases and how phages overcome them, Class 1 OLD family nucleases warrant further study as there has only been one anti-phage Class 1 OLD family nuclease described to date.
View Article and Find Full Text PDFSustainable synthesis of benzimidazole-based Schiff base using reusable CaAlO nanophosphors catalyst: Insights into metal(II) complexes and DNA interactions.
Nucleosides Nucleotides Nucleic Acids
January 2025
Department of PG Studies and Research in Environmental Science, Kuvempu University, Shankaraghatta, India.
This article presents a new and facile method for the synthesis of Schiff base compounds with a benzimidazole group using a low-cost and reusable calcium aluminate nanophosphorus catalyst (CaAlO). This approach avoids harmful solvents and reactants, supporting a more environmentally friendly synthesis process. The catalyst maintained its activity and heterogeneity over four cycles with minimal loss of efficiency.
View Article and Find Full Text PDFClock genes regulate sex pheromone production and male mating ability in Bactrocera dorsalis.
Insect Sci
January 2025
Department of Entomology, College of Plant Protection, South China Agricultural University, Guangzhou, China.
Many animals display physiological and behavioral activities limited to specific times of the day. Certain insects exhibit clear daily rhythms in their mating activities that are regulated by an internal biological clock. However, the specific genetic mechanisms underlying this regulation remain largely unexplored.
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