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The Effect of Reactive Oxygen Species on Respiratory Complex I Activity in Liposomes.
Chemistry
October 2024
Department of Chemistry, Imperial College London, Molecular Sciences Research Hub, White City Campus, London, W12 0BZ, UK.
Respiratory complex I (R-CI) is an essential enzyme in the mitochondrial electron transport chain but also a major source of reactive oxygen species (ROS), which are implicated in neurodegenerative diseases and ageing. While the mechanism of ROS production by R-CI is well-established, the feedback of ROS on R-CI activity is poorly understood. Here, we perform EPR spectroscopy on R-CI incorporated in artificial membrane vesicles to reveal that ROS (particularly hydroxyl radicals) reduce R-CI activity by making the membrane more polar and by increasing its hydrogen bonding capability.
View Article and Find Full Text PDFFluorescence-Based Proteoliposome Methods to Monitor Redox-Active Transition Metal Transmembrane Translocation by Metal Transporters.
Methods Mol Biol
July 2024
Department of Chemistry and Biochemistry, The University of Texas at Dallas, Richardson, TX, USA.
Transmembrane transition metal transporter proteins are central gatekeepers in selectively controlling vectorial metal cargo uptake and extrusion across cellular membranes in all living organisms, thus playing key roles in essential and toxic metal homeostasis. Biochemical characterization of transporter-mediated translocation events and transport kinetics of redox-active metals, such as iron and copper, is challenged by the complexity in generating reconstituted systems in which vectorial metal transport can be studied in real time. We present fluorescence-based proteoliposome methods to monitor redox-active metal transmembrane translocation upon reconstitution of purified metal transporters in artificial lipid bilayers.
View Article and Find Full Text PDFThe Structural and Spectral Features of Light-Harvesting Complex II Proteoliposomes Mimic Those of Native Thylakoid Membranes.
J Phys Chem Lett
June 2022
Department of Biochemistry, School of Biological and Behavioural Sciences, Queen Mary University of London, Mile End Road, London E1 4NS, United Kingdom.
The major photosystem II light-harvesting antenna (LHCII) is the most abundant membrane protein in nature and plays an indispensable role in light harvesting and photoprotection in the plant thylakoid. Here, we show that "pseudothylakoid characteristics" can be observed in artificial LHCII membranes. In our proteoliposomal system, at high LHCII densities, the liposomes become stacked, mimicking the thylakoid grana membranes.
View Article and Find Full Text PDFProteoliposome-Incorporated Seawater Reverse Osmosis Polyamide Membrane: Is the Aquaporin Water Channel Effect in Improving Membrane Performance Overestimated?
Environ Sci Technol
April 2022
Singapore Membrane Technology Centre, Nanyang Environment and Water Research Institute, Nanyang Technological University, 1 Cleantech Loop, Singapore 637141, Singapore.
The water channel feature of the aquaporin (AQP) is considered to be the key in improving the permselectivity of AQP-based thin-film composite (TFC) polyamide (PA) membranes, yet much less attention has been paid to the physicochemical property changes of the PA layer induced by AQP-reconstituted proteoliposomes. This study systematically investigated the roles of proteoliposome constituents (liposome/detergent/AQP) in affecting the physicochemical properties and performance of the membranes. For the first time, we demonstrated that the constituents in the proteoliposome could facilitate the formation of a PA layer with enlarged protuberances and thinner crumples, resulting in a 79% increase in effective surface area and lowering of hydraulic resistance for filtration.
View Article and Find Full Text PDFArtificial double inversion recovery images can substitute conventionally acquired images: an MRI-histology study.
Sci Rep
February 2022
Department of Anatomy and Neurosciences, MS Center Amsterdam, Amsterdam Neurosciences, Amsterdam UMC, Vrije Universiteit Amsterdam, De Boelelaan 1117, 1081 HV, Amsterdam, The Netherlands.
Cortical multiple sclerosis lesions are disease-specific, yet inconspicuous on magnetic resonance images (MRI). Double inversion recovery (DIR) images are sensitive, but often unavailable in clinical routine and clinical trials. Artificially generated images can mitigate this issue, but lack histopathological validation.
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