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Biological characterization of breast cancer spheroid formed by fast fabrication method.
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February 2024
Faculty of Engineering, Tokyo University of Agriculture and Technology, 2-24-16 Naka-Cho, Koganei, Tokyo 184-8588 Japan.
Unlabelled: Engineered three-dimensional (3D) tissue culture platforms are useful for reproducing and elucidating complex in vivo biological phenomena. Spheroids, 3D aggregates of living cells, are produced based on physicochemical or microfabrication technologies and are commonly used even in cancer pathology research. However, conventional methods have difficulties in constructing 3D structures depending on the cell types, and require specialized techniques/lab know-how to reproducibly control the spheroid size and shape.
View Article and Find Full Text PDFA case of bacteremia in a 38-year-old homeless male originating from a maggot-infested amputated foot.
IDCases
January 2025
University of Kansas Medical Center, Department of Internal Medicine, Division of Infectious Diseases, 3901 Rainbow Blvd., Mailstop 1028, Kansas City, KS 66160, USA.
() was first isolated from the larval stage of the fly vector It is a gram-negative, non-motile, strictly aerobic rod that thrives in temperatures between 28º C and 37º C. Its strong chitinase activity aids in metamorphosis, which suggests a symbiotic relationship with the fly. Although rare, has been implicated in human infections, like bacteremia and osteomyelitis, typically transmitted through fly larvae in skin wounds.
View Article and Find Full Text PDFAmino acid assisted synthesis of CDs: a novel paradigm in plant tissue culture media for enhanced cellular effects and biotechnological advancements.
RSC Adv
January 2025
Centre for Nano Bio Polymer Science and Technology, Department of Physics, St. Thomas College Palai Kerala 686574 India +919446126926.
We report a green approach to prepare carbon dots (CDs) with fresh tomatoes as carbon sources and amino acids as dopants (ACDs) by a microwave assisted method. The synthesised CDs were analysed by UV-visible absorption spectroscopy, photoluminescence spectroscopy, high resolution transmission electron spectroscopy, X-ray diffraction, Fourier transform infrared spectroscopy, X-ray photo electron spectroscopy. An MTT assay was used to evaluate the cytotoxicity of CDs toward L929 cells and found that CDs exhibit low cytotoxicity.
View Article and Find Full Text PDFAdipo-on-chip: a microphysiological system to culture human mesenchymal stem cells with improved adipogenic differentiation.
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December 2024
Laboratório de Biologia Básica de Células-Tronco, FIOCRUZ, Rua Professor Algacyr Munhoz Mader, 3775, Instituto Carlos Chagas, Curitiba, Paraná PR 81350-010 Brazil.
Obesity is associated with several comorbidities that cause high mortality rates worldwide. Thus, the study of adipose tissue (AT) has become a target of high interest because of its crucial contribution to many metabolic diseases and metabolizing potential. However, many AT-related physiological, pathophysiological, and toxicological mechanisms in humans are still poorly understood, mainly due to the use of non-human animal models.
View Article and Find Full Text PDFHybrid additive manufacturing for Zn-Mg casting for biomedical application.
In Vitro Model
December 2024
Department of Industrial and Manufacturing Engineering, Pennsylvania State University, State College, University Park, PA USA.
Zinc (Zn) and its alloys have been the focus of recent materials and manufacturing research for orthopaedic implants due to their favorable characteristics including desirable mechanical strength, biodegradability, and biocompatibility. In this research, a novel process involving additive manufacturing (AM) augmented casting was employed to fabricate zinc-magnesium (Zn-0.8 Mg) artifacts with surface lattices composed of triply periodic minimal surfaces (TPMS), specifically gyroid.
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