Insoluble polyelectrolytes (PE60) were used for the concentration of viruses from stool specimens, confirming the results of Wallis et al. (1969). Ten percent suspensions inoculated with poliovirus type 3 were used in these experiments. A small number of stool specimens from patients naturally infected with enteroviruses were also tested. Preferential adsorption of viruses to PE60 was maximum at a pH range of 4.5 to 6.0. The elution of the adsorbed viruses was optimal at pH 8.5. Other parameters were also investigated. Electron microscopy was used successfully to detect the eluted viruses.
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http://dx.doi.org/10.1128/am.24.2.270-274.1972 | DOI Listing |
J Orthop Surg Res
January 2025
Department of Joint Osteopathy, Liuzhou Worker's Hospital, Liuzhou, Guangxi Province, 545000, China.
Alcoholic osteonecrosis of the femoral head (AIONFH) is caused by long-term heavy drinking, which leads to abnormal alcohol and lipid metabolism, resulting in femoral head tissue damage, and then pathological necrosis of femoral head tissue. If not treated in time in clinical practice, it will seriously affect the quality of life of patients and even require hip replacement to treat alcoholic femoral head necrosis. This study will confirm whether M2 macrophage exosome (M2-Exo) miR-122 mediates alcohol-induced BMSCs osteogenic differentiation, ultimately leading to the inhibition of femoral head necrosis.
View Article and Find Full Text PDFCardiovasc Diabetol
January 2025
Department of Cardiology, Beijing Anzhen Hospital, Capital Medical University, No.2 Anzhen Road, Chaoyang District, 100029, Beijing, China.
Introduction: Bone marrow-derived mesenchymal stem cell-derived extracellular vesicles (BMSC-EVs) are widely used for therapeutic purposes in preclinical studies. However, their utility in treating diabetes-associated atherosclerosis remains largely unexplored. Here, we aimed to characterize BMSC-EV-mediated regulation of autophagy and macrophage polarization.
View Article and Find Full Text PDFBMC Plant Biol
January 2025
Plant Production Department, College of Food and Agricultural Sciences, King Saud University, P.O. Box. 2460, Riyadh, 11451, Saudi Arabia.
Background: The present research work was done to evaluate the anatomical differences among selected species of the family Bignoniaceae, as limited anatomical data is available for this family in Pakistan. Bignoniaceae is a remarkable family for its various medicinal properties and anatomical characterization is an important feature for the identification and classification of plants.
Methodology: In this study, several anatomical structures were examined, including stomata type and shape, leaf epidermis shape, epidermal cell size, and the presence or absence of trichomes and crystals (e.
BMC Nephrol
January 2025
Department of Nephrology, Japan Community Healthcare Organization Sendai Hospital, 981-3281, Sendai, Miyagi, Japan.
Background: Oliguric acute kidney injury (AKI) is one of the critical conditions which needs emergent treatment due to the lack of the capacity of excreting toxins and fluids, and plasma membrane bleb formation is considered as one of the characteristic morphologic alterations in ischemic AKI in both animal models and human. We present here an autopsy case with clear electron microscopy images capturing a definitive instance of blebbing in ischemic AKI.
Case Presentation: A 66-year-old man was admitted for oliguric AKI with nephrotic syndrome (NS).
BMC Microbiol
January 2025
Department of Internal Medicine and Infectious Diseases (Infectious Diseases), Faulty of Veterinary Medicine, Cairo University, Giza, Egypt.
Background: The excessive use of antibiotics is a major contributor to the global issue of antimicrobial resistance (AMR), a significant threat to human and animal health. Hence, assessing new strategies for managing Multi-Drug Resistant (MDR) microorganisms is vital. In this study, the use of mechanically isolated mature adipose cells (MIMACs) and their lysate (Adipolysate) as a new sustainable antimicrobial agent was assessed against Methicillin-resistant Staphylococcus aureus (MRSA).
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