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Differentiation of and by Metabolite Profiles Obtained Using Gold Nanoparticles-Based Surface-Assisted Laser Desorption/Ionization Mass Spectrometry.
Pathogens
December 2024
Department of Inorganic and Analytical Chemistry, Faculty of Chemistry, Rzeszów University of Technology, Powstańców Warszawy 6, 35-959 Rzeszów, Poland.
and are challenging to differentiate using methods such as phenotyping, 16S rRNA sequencing, or protein profiling through matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) due to their close relatedness. This study explores the potential for identifying and by incorporating reference spectra of metabolite profiles, obtained via surface-assisted laser desorption/ionization mass spectrometry (SALDI MS) employing gold nanoparticles (AuNPs), into the Bruker Biotyper database. Metabolite extracts from and cells were prepared using liquid-liquid extraction in a chloroform-methanol-water system.
View Article and Find Full Text PDFExploring the Mechanism of Protein for Broiler Growth Based on Gut Microbiota and Serum Metabolomics.
Biology (Basel)
January 2025
School of Biological Engineering, Dalian Polytechnic University, Dalian 116034, China.
Intestinal health is vital for poultry production, and protein plays a key role in intestinal nutrition. The present study used 16S rRNA gene sequencing and serum metabolomics to investigate the effect of CAP on the cecal microflora structure and serum metabolites in 42-day-old broiler chickens. A total of 480 one-day-old Arbor Acres broiler chickens were randomly divided into four treatments with twelve replicates comprising 10 chickens each, evenly divided by sex.
View Article and Find Full Text PDFDiacylation of Peptides Enables the Construction of Functional Vesicles for Drug-Carrying Liposomes.
Angew Chem Int Ed Engl
January 2025
University of California, San Diego, Chemistry and Biochemistry, 9500 Gilman Drive, Urey Hall 4120, 92093, La Jolla, UNITED STATES OF AMERICA.
Membrane-forming phospholipids are generated in cells by enzymatic diacylation of non-amphiphilic polar head groups. Analogous non-enzymatic processes may have been relevant at the origin of life and could have practical utility in membrane synthesis. However, aqueous head group diacylation is challenging in the absence of enzymes.
View Article and Find Full Text PDFAntimicrobial Nanoparticles Composed of Zein and Arginine-Phenylalanine-Based Surfactants for Wound Related Infections: Antioxidant and Skin-Related Anti-Enzymatic Activities and Toxicity.
Antibiotics (Basel)
December 2024
Department of Surfactants and Nanobiotechnology, Instituto de Química Avanzada de Cataluña, Centro Superior de Investigaciones Científicas IQAC-CSIC, 08035 Barcelona, Spain.
: Cationic surfactants are potential antimicrobial candidates. Even so, they are the foremost irritative and incompatible group, which limits their usage. The incorporation of surfactants in biopolymer-based nanoparticles is a feasible strategy to improve their efficacy and reduce those drawbacks.
View Article and Find Full Text PDFComparison of blending and bonding of phytic acid arginine salt and cellulose nanofibers on their synergistic flame-retardant effect in poly (butylene succinate).
Int J Biol Macromol
January 2025
School of Light Industry Science and Engineering, Beijing Technology and Business University, Beijing 100048, China; China Advanced Flame Retardant Engineering Technology Research Center for Light Industry, Beijing 100048, China; Engineering laboratory for halogen-free flame retardants for polymer materials in the petroleum and chemical industry, Beijing 100048, China.
In this study, cellulose nanofibers (CNFs) were utilized as a synergistic agent, and combined with phytic acid arginine salt (PaArg) via blending and bonding. The effects of these different binding techniques of CNFs and PaArg on the flame retardant and mechanical properties of poly (butylene succinate) (PBS) were explored. The results indicated that both blended and bonded CNFs and PaArg enabled PBS composites to achieve a UL 94 V-0 rating, with the limiting oxygen index (LOI) value of the composite exceeding 28 %.
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