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Complex transcription regulation of acidic chitinase suggests fine-tuning of digestive processes in Drosera binata.
Planta
January 2025
Institute of Plant Genetics and Biotechnology, Plant Science and Biodiversity Center, Slovak Academy of Sciences, Akademicka 2, P. O. Box 39A, 950 07, Nitra, Slovak Republic.
DbChitI-3, Drosera binata's acidic chitinase, peaks at pH 2.5 from 15 °C to 30 °C. Gene expression is stimulated by polysaccharides and suppressed by monosaccharide digestion, implying a feedback loop in its transcriptional regulation.
View Article and Find Full Text PDFExcited state intramolecular proton transfer (ESIPT) termination enables "one-to-two" sensing property of an indolocarbazole-based Schiff base.
Spectrochim Acta A Mol Biomol Spectrosc
December 2024
School of Chemistry and Chemical Engineering, Guizhou University, Guiyang 550025, PR China. Electronic address:
Detections towards multiple analytes by one sensor remain a significant challenges. Herein, a high-sensitivity chemo-sensor ICZ-o-XFJ is designed and synthesized for the simultaneous detection of F and Al. Suffering from the ESIPT process, this sensor is colorless and almost non-emissive in solutions.
View Article and Find Full Text PDFEvaluation of carp sperm respiration: fluorometry with optochemical oxygen sensor versus polarography.
Fish Physiol Biochem
February 2025
South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in České Budějovice, Zátiší 728/II, 389 25, Vodňany, Czech Republic.
The primary function of spermatozoa is to fertilize the oocyte, which depends on their motility and is directly associated with their metabolic state. The oxygen consumption rate (OCR) of spermatozoa reflects the respiratory capacity of sperm mitochondria under various physiological conditions and is an essential marker of sperm quality. We determined the OCR of common carp (Cyprinus carpio) sperm using two respirometry methods: the conventionally used polarographic method with a Clark-type electrode and fluorometric assay with an Oxo Dish optochemical oxygen sensor.
View Article and Find Full Text PDFDopamine-supported HPLC post-column derivatization to fluorescence: Simultaneous and sensitive detection of eight tea polyphenols.
Food Chem
February 2025
College of Chemistry, Beijing University of Chemical Technology, Beijing 100029, China. Electronic address:
The effective differentiation and detection of multiple tea polyphenols are often challenging due to their subtle structural similarities. Although post-column derivatization HPLC strategies are commonly employed to distinguish multiple targets, the short physical distance between chromatographic column and detector limits reaction time, thereby reducing the derivatization efficiency. Dopamine (DA) reacts rapidly with resorcinol to form fluorescent azamonardine products, making fast fluorometric derivatization of tea polyphenols containing resorcinol motifs possible.
View Article and Find Full Text PDFA ratiometric fluorescence nanosensor for glutathione detection based on spatially confined dual-emission of α-lipoic acid-modified gold nanoclusters and silicon nanoparticles.
Anal Methods
November 2024
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Tabuk, Tabuk 71491, Saudi Arabia.
The development of dual-emission ratiometric fluorescent probes with aggregation-induced emission enhancement (AIEE) overcomes the limitations of gold nanocluster (Au NC)-based probes, particularly their weak intrinsic fluorescence, in real-world applications. These AIEE probes also exhibit superior detection limits and enhanced sensitivity. A novel combination for the reliable fluorometric detection of glutathione (GSH) was proposed, utilizing aggregation-induced emission enhancement (AIEE) facilitated by electrostatic interaction and spatial confinement.
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