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Identification of Proteoforms Related to Flower Petaloid Through Proteogenomic Strategy.
Proteomes
January 2025
State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan 430026, China.
is an aquatic plant with a high ornamental value due to its flower. Despite the release of several versions of the lotus genome, its annotation remains inefficient, which makes it difficult to obtain a more comprehensive knowledge when -omic studies are applied to understand the different biological processes. Focusing on the petaloid of the lotus flower, we conducted a comparative proteomic analysis among five major floral organs.
View Article and Find Full Text PDFEvaluation of 16S rRNA genes sequences and genome-based analysis for identification of non-pathogenic .
Front Microbiol
January 2025
Laboratory for Plague Microbiology, Especially Dangerous Infections Department, State Research Center for Applied Microbiology and Biotechnology, Obolensk, Russia.
16S rRNA genes sequencing has been used for routine species identification and phylogenetic studies of bacteria. However, the high sequence similarity between some species and heterogeneity within copies at the intragenomic level could be a limiting factor of discriminatory ability. In this study, we aimed to compare 16S rRNA genes sequences and genome-based analysis (core SNPs and ANI) for identification of non-pathogenic .
View Article and Find Full Text PDFA scalable CRISPR-Cas9 gene editing system facilitates CRISPR screens in the malaria parasite Plasmodium berghei.
Nucleic Acids Res
January 2025
The Laboratory for Molecular Infection Medicine Sweden, Umeå University, Försörjningsvägen 2A, 901 87 Umeå, Sweden.
Many Plasmodium genes remain uncharacterized due to low genetic tractability. Previous large-scale knockout screens have only been able to target about half of the genome in the more genetically tractable rodent malaria parasite Plasmodium berghei. To overcome this limitation, we have developed a scalable CRISPR system called P.
View Article and Find Full Text PDFGOPhage: protein function annotation for bacteriophages by integrating the genomic context.
Brief Bioinform
November 2024
Department of Electrical Engineering, City University of Hong Kong, 83 Tat Chee Ave, Kowloon Tong, Hong Kong (SAR), China.
Bacteriophages are viruses that target bacteria, playing a crucial role in microbial ecology. Phage proteins are important in understanding phage biology, such as virus infection, replication, and evolution. Although a large number of new phages have been identified via metagenomic sequencing, many of them have limited protein function annotation.
View Article and Find Full Text PDFGenome-wide identification of MGT gene family in soybean (Glycine max) and their expression analyses under magnesium stress conditions.
BMC Plant Biol
January 2025
Faculty of Biotechnology, October University for Modern Sciences & Arts, 6th October City, Egypt.
Background: Magnesium (Mg) is essential for plant growth and development and plays critical roles in physiological and biochemical processes. Mg deficiency adversely affects growth of plants by limiting shoot and root development, disturbing the structure and membranes of the grana, reducing photosynthesis efficiency, and lowering net CO assimilation. The MGT (Magnesium transporter) family is responsible for the absorption and transportation of magnesium in plants.
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