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Piezoelectric biosensor with dissipation monitoring enables the analysis of bacterial lytic agent activity.
Sci Rep
January 2025
Department of Biochemistry, Faculty of Science, Masaryk University, Kamenice 5, 625 00, Brno, Czech Republic.
Antibiotic-resistant strains of Staphylococcus aureus pose a significant threat in healthcare, demanding urgent therapeutic solutions. Combining bacteriophages with conventional antibiotics, an innovative approach termed phage-antibiotic synergy, presents a promising treatment avenue. However, to enable new treatment strategies, there is a pressing need for methods to assess their efficacy reliably and rapidly.
View Article and Find Full Text PDFCombinatorial phenotypic landscape enables bacterial resistance to phage infection.
bioRxiv
January 2025
Institute for Systems Biology, Seattle, WA, USA.
Success of phage therapies is limited by bacterial defenses against phages. While a large variety of anti-phage defense mechanisms has been characterized, how expression of these systems is distributed across individual cells and how their combined activities translate into protection from phages has not been studied. Using bacterial single-cell RNA sequencing, we profiled the transcriptomes of ~50,000 cells from cultures of a human pathobiont, , infected with a lytic bacteriophage.
View Article and Find Full Text PDFAn insight into the applications of bacteriophages against food-borne pathogens.
J Food Sci Technol
January 2025
Department of Genetic Engineering, Faculty of Engineering and Technology, School of Bioengineering, SRM Institute of Science and Technology, SRM Nagar, Kattankulathur, Kanchipuram, Chennai, Tamil Nadu 603203 India.
Novel and emerging pathogens, enduring contamination, antibiotic resistance, an environment that is always changing, and the complexity of food production systems all contribute to the worsening of foodborne illness. It has been proposed that bacteriophages can serve as both fast food-borne pathogen detection tools and natural food preservatives in a variety of foods. Phages, like many other antimicrobial interventions used in food production systems, are not a cure-all for issues related to food safety, though.
View Article and Find Full Text PDFEvaluation of the Effect of Bacteriophages and Organic Acids as a Feed Additive to Reduce Salmonella enteritidis in Challenged Chickens.
J Anim Physiol Anim Nutr (Berl)
January 2025
Department Animal Science, Higher Education Complex of Torbat-e Jam, Torbat-e Jam, Iran.
This study aimed to compare the effects of dietary supplementation of bacteriophage (BP) and acidifiers on performance, meat quality, morphology, and intestinal microbiota in chickens challenged and unchallenged with Salmonella enteritidis (SE) and also to investigate the possibility of replacing them in the diet with antibiotics. A total of 1760 male Ross (308) chicks were randomly assigned to 11 dietary treatments (8 pens/with 20 male chickens in each). Dietary treatments were as follows: SE-uninfected (negative control (NC), a basal diet without supplemention; NC+ 500 g/t BP (NBP1); NC+ 1000 g/t BP (NBP2); NC+ 300 mg/kg acidifier A (NAA); NC+ 300 mg/kg acidifier B (NAB)) and SE-infected (positive control (PC), a basal diet without supplemention; PC+ 40 mg/kg Antibiotic enrofloxacin (PA); PC+ 500 g/t BP (PBP1); PC+ 1000 g/t BP (PBP2); PC+ 3000 mg/kg acidifier A (PAA); PC+ 3000 mg/kg acidifier B (PAB)).
View Article and Find Full Text PDFEngineered protein G variants for multifunctional antibody-based assemblies.
Protein Sci
February 2025
Department of Biochemistry and Molecular Biology, The University of Chicago, Chicago, Illinois, USA.
We have developed a portfolio of antibody-based modules that can be prefabricated as standalone units and snapped together in plug-and-play fashion to create uniquely powerful multifunctional assemblies. The basic building blocks are derived from multiple pairs of native and modified Fab scaffolds and protein G (PG) variants engineered by phage display to introduce high pair-wise specificity. The variety of possible Fab-PG pairings provides a highly orthogonal system that can be exploited to perform challenging cell biology operations in a straightforward manner.
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