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Bridging the Gap: Phage Manufacturing Processes from Laboratory to Agri-Food Industry.
Virus Res
January 2025
Department of Genomics, Branch for Northwest & West Region, Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education and Extension Organization (AREEO), Tabriz, Iran. Electronic address:
Interest in bacteriophages (phages) as sustainable biocontrol agents in the agri-food industry has increased because of growing worries about food safety and antimicrobial resistance (AMR). The phage manufacturing process is examined in this review, with particular attention paid to the crucial upstream and downstream processes needed for large-scale production. Achieving large phage yields requires upstream procedures, including fermentation and phage amplification.
View Article and Find Full Text PDFIsolation, characterization, and genome sequencing analysis of a novel phage HBW-1 of Salmonella.
Microb Pathog
January 2025
Laboratory of Molecular Microbiology and Food Safety, Zhejiang University College of Animal Sciences, Hangzhou, 310058, China; Hainan Institute of Zhejiang University, Sanya, 572025, China; Key Laboratory of Systems Health Science of Zhejiang Province, School of Life Science, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Hangzhou, 310024, China. Electronic address:
Salmonella presents a significant threat to the health of animals and humans, especially with the rise of strains resistant to multiple drugs. This highlights the necessity for creating sustainable and efficient practical approaches to managing salmonellosis. The most recent and safest approach to combat antimicrobial resistance-associated infections is lytic bacteriophages.
View Article and Find Full Text PDFBiochemistry of Bacterial Biofilm: Insights into Antibiotic Resistance Mechanisms and Therapeutic Intervention.
Life (Basel)
January 2025
Medicinal Chemistry Laboratory, Department of Biosciences, Jamia Millia Islamia, Jamia Nagar, New Delhi 110025, India.
Biofilms, composed of structured communities of bacteria embedded in a self-produced extracellular matrix, pose a significant challenge due to their heightened resistance to antibiotics and immune responses. This review highlights the mechanisms underpinning antibiotic resistance within bacterial biofilms, elucidating the adaptive strategies employed by microorganisms to withstand conventional antimicrobial agents. This encompasses the role of the extracellular matrix, altered gene expression, and the formation of persister cells, contributing to the recalcitrance of biofilms to eradication.
View Article and Find Full Text PDFA phage amplification-assisted SEA-CRISPR/Cas12a system for viable bacteria detection.
J Mater Chem B
January 2025
Key Laboratory of Medical Diagnostics of Ministry of Education, College of Laboratory Medicine, Chongqing Medical Laboratory Microfluidics and SPRi Engineering Research Center, Chongqing Medical University, Chongqing, 400016, P. R. China.
Rapid and accurate detection of viable bacteria is essential for the clinical diagnosis of urinary tract infections (UTIs) and for making effective therapeutic decisions. However, most current molecular diagnostic techniques are unable to differentiate between viable and non-viable bacteria. In this study, we introduce a novel isothermal platform that integrates strand exchange amplification (SEA) with the CRISPR/Cas12a system, thereby enhancing both the sensitivity and specificity of the assay and achieving detection of phage DNA at concentrations as low as 4 × 10 copies per μL.
View Article and Find Full Text PDFPhage-encoded depolymerases as a strategy for combating multidrug-resistant .
Front Cell Infect Microbiol
November 2024
Department of Microbiology, College of Science and Technology, Dankook University, Cheonan, Republic of Korea.
Article Synopsis
- A nosocomial pathogen poses a significant public health risk due to its resistance to multiple antibiotics, making treatment of severe infections difficult and often leading to high mortality rates.
- The potential use of bacteriophage-derived polysaccharide depolymerase enzymes is highlighted as a novel approach, targeting bacterial components to disrupt biofilms and enhance treatment effectiveness.
- However, challenges like the narrow host range of these enzymes and the emergence of phage-resistant strains necessitate further research and clinical trials to evaluate their full potential in therapeutic applications.
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