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Conformational flexibility of human ribokinase captured in seven crystal structures.
Int J Biol Macromol
January 2025
Department of Biochemistry, Memorial University of Newfoundland, 45 Arctic Avenue, St. John's, Newfoundland and Labrador, Canada. Electronic address:
d-ribose is a critical sugar substrate involved in the biosynthesis of nucleotides, amino acids, and cofactors, with its phosphorylation to ribose-5-phosphate by ribokinase (RK) constituting the initial step in its metabolism. RK is conserved across all domains of life, and its activity is significantly enhanced by monovalent metal (M) ions, particularly K, although the precise mechanism of this activation remains unclear. In this study, we present several crystal structures of human RK in both unliganded and substrate-bound states, offering detailed insights into its substrate binding process, reaction mechanism, and conformational changes throughout the catalytic cycle.
View Article and Find Full Text PDFEnantiodivergent Synthesis of Both ()- and ()-Heteroaryl Aldols by Rationally Engineered Aldolases.
J Org Chem
January 2025
School of Chemistry, University of Hyderabad, Hyderabad 500 046, Telangana, India.
Aldolases, especially 2-deoxyribose-5-phosphate aldolase (DERA) enzymes, have been widely employed to access key chiral precursors for various active pharmaceutical ingredients (APIs). This has been enabled by expanding their substrate scope toward non-natural acceptors and donors via protein engineering. In this study, we endeavored to broaden the acceptor substrate scope of DERA from sp.
View Article and Find Full Text PDFA highly sensitive photoelectrochemical sensor for the detection of theophylline based on the photoelectrocatalytic activity of a nanocomposite of polydopamine nanospheres and gold nanoparticles.
Anal Methods
January 2025
School of Pharmacy, Wannan Medical College, Wuhu 241002, China.
A label-free photoelectrochemical (PEC) sensor for detecting theophylline (TP) was exploited based on electrodes modified with a nanocomposite of polydopamine nanospheres (PDSs) and gold nanoparticles (AuNPs). PDS particles were prepared by oxidative autopolymerization, and their reducibility was utilized in one step to reduce the gold nanoparticles . The AuNPs-PDS/ZnS PEC sensor was constructed by electrochemical deposition and drop coating.
View Article and Find Full Text PDFPI5P4K inhibitors: promising opportunities and challenges.
FEBS J
January 2025
Institute for Advanced Biosciences, Keio University, Tsuruoka, Japan.
Phosphatidylinositol 5-phosphate 4-kinases (PI5P4K), also known as type II PIPKs or PIPKIIs, convert the lipid second messenger PI5P to PI(4,5)P. The PI5P4K family consists of three isozymes in mammals-PI5P4Kα, β, and γ-which notably utilize both GTP and ATP as phosphodonors. Unlike the other two isozymes, which can utilize both ATP and GTP, PI5P4Kβ exhibits a marked preference for GTP over ATP, acting as an intracellular GTP sensor that alters its kinase activity in response to physiological changes in GTP concentration.
View Article and Find Full Text PDFRapid, flexible fabrication of a microfluidic electrochemical chip nucleic acid target for selective, label-free detection of influenza virus DNA using catalytic redox-recycling.
Anal Biochem
January 2025
Department of Analytical Chemistry, Faculty of Chemistry, Razi University, Kermanshah, Iran.
H5N1 flu is a highly virulent and variable subtype of influenza with significant epidemic and pandemic potential. In this study, we introduce a novel, maskless, and rapid manufacturing process for a microfluidic chip integrated with electrodes for the quantitative detection of H5N1-DNA sequences. This detection leverages a catalytic redox-recycling signal via a novel Fe₃O₄@TMU-8 nanocomposite, which facilitates the turnover of the oxidation state of [Ru(NH₃)₆]³⁺, thereby amplifying the electrochemical signal output.
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