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The quality of biological samples used in metabolomics research is significantly influenced by preanalytical factors, such as the timing of centrifugation and freezing. This study aimed to evaluate how preanalytical factors, like delays in centrifugation and freezing, affect metabolomics research. Blood samples, collected in various tube types, were subjected to controlled pre- and postcentrifugation delays.
View Article and Find Full Text PDFAnalysis of Seroreactivity and Seropositivity in Balb/c Mice Experimentally Infected with Toxocara canis Using Two Recombinant (rTc-CTL-1 and rTES-120) Antigens.
Acta Parasitol
January 2025
Centralized Instrumentation Laboratory, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai, 600 007, India.
Introduction: Toxocarosis in human beings is currently diagnosed by serological assay based on the detection of antibodies against Toxocara antigens. Toxocara canis larvae do not reach the adult stage in paratenic hosts like humans and mice. Therefore experimental infection in mice, which mimics the biology of human infection, might be relevant to get a better understanding of human toxocarosis.
View Article and Find Full Text PDFSwarmMAP: Swarm Learning for Decentralized Cell Type Annotation in Single Cell Sequencing Data.
bioRxiv
January 2025
Else Kroener Fresenius Center for Digital Health, Technical University Dresden, Fetscherstraße 74, Dresden, 01307, Saxony, Germany.
Rapid technological advancements have made it possible to generate single-cell data at a large scale. Several laboratories around the world can now generate single-cell transcriptomic data from different tissues. Unsupervised clustering, followed by annotation of the cell type of the identified clusters, is a crucial step in single-cell analyses.
View Article and Find Full Text PDFIn-situ engineering of centralized mesopores and edge nitrogen for porous carbons toward zinc ion hybrid capacitors.
J Colloid Interface Sci
January 2025
School of Chemistry and Chemical Engineering, Guangxi Key Laboratory of Petrochemical Resource Processing and Process Intensification Technology, Guangxi University (GXU), 100 Daxuedong Road, Xixiangtang District, Nanning 530004 China. Electronic address:
Porous carbons with large surface area (>3000 m/g) and heteroatom dopants have shown great promise as electrode materials for zinc ion hybrid capacitors. Centralized mesopores are effective to accelerate kinetics, and edge nitrogen can efficiently enhance pseudocapacitive capability. It is a great challenge to engineer centralized mesopores and edge nitrogen in large-surface-area porous carbons.
View Article and Find Full Text PDFSample Tracking Tool: A Comprehensive Approach Based on OpenArray Technology and R Scripting for Genomic Sample Monitoring.
Diagnostics (Basel)
January 2025
Genomic Medicine Laboratory UILDM, IRCCS Santa Lucia Foundation, 00179 Rome, Italy.
: Centralizing genetic sequencing in specialized facilities is pivotal for reducing the costs associated with diagnostic testing. These centers must be able to verify data quality and ensure sample integrity. This study aims at developing a protocol for tracking NGS-analyzed samples to prevent errors and mix-ups, ensuring proper quality control, accuracy, and reliability in genetic testing procedures.
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