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The carbohydrate sulfotransferase 6 (chst6) gene is linked to macular corneal dystrophy (MCD), a rare disease that leads to bilateral blindness due to the accumulation of opaque aggregates in the corneal stroma. chst6 encodes for a keratan sulfate proteoglycan (KSPG) specific sulfotransferase. MCD patients lose sulfated KSPGs (cKS) in the cornea and the serum.
View Article and Find Full Text PDFRole of sulfated GAGs in shear mechanical properties of human and porcine cornea.
Exp Eye Res
December 2024
Mechanical and Industrial Engineering Department, University of Illinois Chicago, Chicago, IL, USA.
Article Synopsis
- - The study examined how glycosaminoglycans (GAGs) affect the viscoelastic properties of corneal tissue from humans and pigs, aiming to inform new treatments for conditions linked to GAG and proteoglycan changes.
- - Researchers used an enzyme to reduce GAG content in corneal samples and analyzed their structure and mechanical properties through specific assays and rheometry.
- - Findings showed that GAG depletion led to a notable increase in interfibrillar spacing, while the shear stiffness of the corneal samples was influenced by the applied strain; this research highlighted the significant role of GAGs in the mechanical behavior of corneal tissues.
Matrix glycosaminoglycans and proteoglycans in human cornea organoids and similarities with fetal corneal stages.
Ocul Surf
November 2024
Department of Ophthalmology, NYU Grossman School of Medicine, Science Building, Fifth Floor 435 E 30th, New York, NY, USA; Department of Pathology, NYU Grossman School of Medicine, New York, NY, USA. Electronic address:
Purpose: We developed human cornea organoids (HCOs) from induced pluripotent stem cells (iPSCs) where single-cell RNA-sequence (scRNA-seq) analysis suggested similarity with developing rather than mature human corneas. We performed immunohistology to determine the presence of corneal glycosaminoglycans as an assessment of maturity. We undertook a detailed comparison of the HCO scRNA-seq data with a recent scRNA-seq study of human fetal corneas at different stages to gauge the HCO's maturity.
View Article and Find Full Text PDFGelatin nanofibers coated with hyaluronic acid as a mesenchymal stromal cell scaffold for corneal regeneration.
Int J Pharm
January 2025
Byers Eye Institute, Department of Ophthalmology at Stanford University School of Medicine, Palo Alto 94304, CA, USA; VA Palo Alto Health Care System, Palo Alto 94304, CA, USA; Department of Chemical Engineering, Stanford University, Stanford 94305, CA, USA. Electronic address:
Electrospun gelatin nanofibers coated with hyaluronic acid (GelNF-HA) were synthesized as a scaffold for delivering human corneal mesenchymal stromal cells (C-MSCs) directly to deep corneal injuries. Aligned GelNFs were produced by electrospinning, crosslinked using vapor of glutaraldehyde, coated with HA, and crosslinked with EDC/NHS. The GelNF-HA was characterized by SEM, mechanical, and optical properties.
View Article and Find Full Text PDFComparative study of physicochemical properties on corneal stromal lenticules following four decellularization methods.
Exp Eye Res
December 2024
Eye Institute and Department of Ophthalmology, Eye & ENT Hospital, Fudan University, Shanghai, 200031, China; Key Laboratory of Myopia and Related Eye Diseases, Chinese Academy of Medical Sciences, Shanghai, 200031, China; Shanghai Research Center of Ophthalmology and Optometry, Shanghai, 200031, China; Shanghai Engineering Research Center of Laser and Autostereoscopic 3D for Vision Care, Shanghai, 200031, China. Electronic address:
This study compares the physicochemical properties of corneal stromal lenticules following decellularization via four methods. Human corneal stromal lenticules, derived from small incision lenticule extraction surgery, underwent decellularization with sodium dodecyl sulfate (SDS), Triton X-100 (Tx) combined with SDS, trypsin-ethylenediaminetetraacetic acid (TE), or NaCl combined with deoxyribonuclease (DNase), respectively. Lenticule DNA and glycosaminoglycan (GAG) content, immunofluorescence staining of cell nuclei and collagen, transparency, biomechanics, histological structure, and immunogenicity were examined in each group and compared with fresh lenticules.
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