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Plants (Basel)
January 2025
Department of Agricultural, Food and Environmental Sciences, Università Politecnica delle Marche (UNIVPM), Via Brecce Bianche 10, 60131 Ancona, Italy.
Water scarcity is an ecological issue affecting over 10% of Europe. It is intensified by rising temperatures, leading to greater evaporation and reduced precipitation. Agriculture has been confirmed as the sector accounting for the highest water consumption globally, and it faces significant challenges relating to drought, impacting crop yields and food security.
View Article and Find Full Text PDFJ Chromatogr A
January 2025
Center of Molecular Immunology, 216 Street and 15th Avenue Atabey-Siboney Playa P.O. Box 16040, Havana, 11600, Cuba. Electronic address:
Protein A chromatography represents the most prevalent methodology for the capture of monoclonal antibodies. The use of a low pH elution buffer from Protein A has been observed to contribute to product aggregation, particularly in the case of IgG4 antibodies, such as nivolumab. This paper presents a well-defined strategy for addressing this issue.
View Article and Find Full Text PDFNPJ Syst Biol Appl
January 2025
The Joint BioEnergy Institute, Lawrence Berkeley National Laboratory, Emeryville, CA, 94608, USA.
Genome-scale metabolic models (GSMM) are commonly used to identify gene deletion sets that result in growth coupling and pairing product formation with substrate utilization and can improve strain performance beyond levels typically accessible using traditional strain engineering approaches. However, sustainable feedstocks pose a challenge due to incomplete high-resolution metabolic data for non-canonical carbon sources required to curate GSMM and identify implementable designs. Here we address a four-gene deletion design in the Pseudomonas putida KT2440 strain for the lignin-derived non-sugar carbon source, p-coumarate (p-CA), that proved challenging to implement.
View Article and Find Full Text PDFJ Environ Manage
January 2025
Department of Life and Environmental Sciences, Università Politecnica Delle Marche, Ancona, Italy.
This study optimized a one-step precipitation process for manganese recovery from a complex medium-bioleachate obtained from electric arc furnace dust (EAFD). The effects of pH variations and different precipitation agents, including acetone, ethanol, oxalic acid, and ammonium hydroxide, were investigated for manganese recovery. While acetone and ethanol facilitated precipitation, they did not lead to the formation of a specific manganese precipitate.
View Article and Find Full Text PDFPlant Physiol Biochem
December 2024
Departamento de Producción Agrícola y Animal, Universidad Autónoma Metropolitana-Xochimilco, Calzada del Hueso 1100, Col. Villa Quietud, 04960, Ciudad de México, Mexico.
Purslane is a plant with high nutritional content that is mainly produced in the central part of Mexico. The nutritional content of purslane depends on various factors such as climatic and soil conditions, phenology, and fertilization. This article describes the H NMR metabolomics profiling of purslane in relation to fertilization at two harvest stages: C and C (27 and 42 days after emergence).
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